<p>In this study, we have reported the use of Ganga water as a solvent for the preparation of soy protein isolate (SPI) films. Generally, distilled water is a solvent of choice for the preparation of protein-based films. Considering the antibacterial and self-purifying capability of Ganga water, we have explored its usage for preparation of biopolymeric i.e., SPI films. The films were casted by solution casting method using 7% SPI w/v and 30% glycerol (with respect to SPI w/w). The distilled water was used as solvent for preparation of control films. Ganga water was filtered through 0.2µ filter to remove bacterial contamination and filtrate was used for the preparation of filtered Ganga water-SPI films. Another film was prepared by taking unfiltered Ganga water as a solvent. The prepared films were characterized by Fourier Transform Infrared (FTIR) spectroscopy and UV-Vis Spectrophotometer for structural and optical barrier properties. The tensile strength and elongation at break of Ganga water incorporated films increased significantly. The Scanning Electron Microscopy images have revealed changes in surface morphology of control and Ganga water incorporated SPI films. The control films demonstrated uniform surface while Ganga water incorporated SPI films had rough and etched surface morphology. Contrary to the expectation, the Ganga water incorporated SPI films showed absence of antibacterial activity against <i>E. coli</i> and <i>L. monocytogenes</i> and that may be due to heating of Ganga water during fabrication of the SPI films. The heating of film solution could have destroyed the heat-labile agents that are responsible for bactericidal activity.</p>