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<p>Morphological descriptors and molecular markers are important for the distinctness, uniformity, and stability (DUS) testing of citrus hybrids. In the present study, we assessed the newly developed interspecific hybrids. Qualitative traits such as leaves, flowers, fruits, and seed characters were assessed in 24 citrus hybrids and four parental genotypes. More than 80% hybrids presented ovate leaf lamina shape, crenate leaf lamina margin with acute leaf lamina apex. A higher phenotypic coefficient of variation (PCV) than genotypic coefficient of variation (GCV) indicates that environmental factors are influencing the expression of studied traits in addition to genetic factors and selection based on phenotype need support by heritability and genetic advance estimates. Notably, pedicel length, fruit weight, and petal width exhibited high PCV and GCV values exceeding 20%. The heritability estimates ranged from 67.25% for leaf lamina length to 99.28% for fruit weight. EST-SSRs cover the entire chromosome 1–9, with the maximum marker generated from chromosome 3 and the minimum from 9. Validation of these markers showed substantial genetic diversity. This investigation into genetic relatedness among the hybrids suggests their potential use in future breeding programs.</p>

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Identification of Newly Developed Interspecific Citrus Hybrids using Morpho-Chemical-Molecular Markers

  • 期刊论文

  • 发表日期：

    2026年5月13日

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  • Raushan Kumar,
  • Nimisha Sharma,
  • Akshay .,
  • A. K. Dubey,
  • R. M. Sharma,
  • Hatkari Vittal,
  • Neha Sharma,
  • Nisha Singh,
  • Mukesh Shivran,
  • Sandeep .,
  • Gyan P. Mishra,
  • Shruti Sethi,
  • Dhrumeshkumar Chavda,
  • Narendra Singh

摘要

Morphological descriptors and molecular markers are important for the distinctness, uniformity, and stability (DUS) testing of citrus hybrids. In the present study, we assessed the newly developed interspecific hybrids. Qualitative traits such as leaves, flowers, fruits, and seed characters were assessed in 24 citrus hybrids and four parental genotypes. More than 80% hybrids presented ovate leaf lamina shape, crenate leaf lamina margin with acute leaf lamina apex. A higher phenotypic coefficient of variation (PCV) than genotypic coefficient of variation (GCV) indicates that environmental factors are influencing the expression of studied traits in addition to genetic factors and selection based on phenotype need support by heritability and genetic advance estimates. Notably, pedicel length, fruit weight, and petal width exhibited high PCV and GCV values exceeding 20%. The heritability estimates ranged from 67.25% for leaf lamina length to 99.28% for fruit weight. EST-SSRs cover the entire chromosome 1–9, with the maximum marker generated from chromosome 3 and the minimum from 9. Validation of these markers showed substantial genetic diversity. This investigation into genetic relatedness among the hybrids suggests their potential use in future breeding programs.