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<p>Efficient RNA isolation is critical for downstream molecular studies, especially in phenolic-rich tissues like mango (<i>Mangifera indica</i> L.). This study evaluated the yield and quality of RNA isolated from mature leaf tissue and flower buds of mango using five Methods. TRIzol, Qiagen kit, Qiagen kit with polyvinylpyrrolidone (PVP), Sigma kit, and Sigma kit with PVP. RNA yield (µg/g tissue) and quality (A260/A280 ratio) varied significantly across methods and tissue types. The TRIzol method yielded the lowest RNA amounts, with 2.85&#xa0;µg/g from mature leaf tissue and 5.60&#xa0;µg/g from flower buds, alongside lowest purity (A260/A280: 1.29–1.48). The Qiagen kit improved yields but remained suboptimal, particularly for flower buds. Incorporating PVP into the Qiagen protocol significantly increased RNA yield (up to 29.85&#xa0;µg/g in mature leaf tissue) and improved quality (A260/A280: 1.83–1.89), highlighting its effectiveness in mitigating phenolic interference. The Sigma kit outperformed other methods, achieving the highest RNA yield (79.83&#xa0;µg/g from mature leaf tissue and 106.26&#xa0;µg/g from flower buds) and excellent purity (A260/A280: 1.86–1.94). Adding PVP to the Sigma kit further enhanced RNA yield, particularly in flower buds (134.18&#xa0;µg/g), while maintaining optimal quality. This study demonstrates the superiority of the Sigma kit, with or without PVP, for extracting high-quality RNA from mango tissues. These findings are valuable for researchers working with phenolic-rich crops, enabling reliable RNA isolation for molecular and transcriptomic analyses.</p>

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Comparison of RNA Isolation Methods in Leaf and Bud of Mango for cDNA Synthesis

  • 期刊论文

  • 发表日期：

    2025年9月10日

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  • Hatkari Vittal,
  • Nimisha Sharma,
  • Kishor Gaikwad,
  • Machindra Nirgude,
  • Suman Lata,
  • Abeer Ali,
  • Neha Sharma,
  • Mukesh Shivran,
  • Sampatirao Dilip,
  • R. M. Sharma

摘要

Efficient RNA isolation is critical for downstream molecular studies, especially in phenolic-rich tissues like mango (Mangifera indica L.). This study evaluated the yield and quality of RNA isolated from mature leaf tissue and flower buds of mango using five Methods. TRIzol, Qiagen kit, Qiagen kit with polyvinylpyrrolidone (PVP), Sigma kit, and Sigma kit with PVP. RNA yield (µg/g tissue) and quality (A260/A280 ratio) varied significantly across methods and tissue types. The TRIzol method yielded the lowest RNA amounts, with 2.85 µg/g from mature leaf tissue and 5.60 µg/g from flower buds, alongside lowest purity (A260/A280: 1.29–1.48). The Qiagen kit improved yields but remained suboptimal, particularly for flower buds. Incorporating PVP into the Qiagen protocol significantly increased RNA yield (up to 29.85 µg/g in mature leaf tissue) and improved quality (A260/A280: 1.83–1.89), highlighting its effectiveness in mitigating phenolic interference. The Sigma kit outperformed other methods, achieving the highest RNA yield (79.83 µg/g from mature leaf tissue and 106.26 µg/g from flower buds) and excellent purity (A260/A280: 1.86–1.94). Adding PVP to the Sigma kit further enhanced RNA yield, particularly in flower buds (134.18 µg/g), while maintaining optimal quality. This study demonstrates the superiority of the Sigma kit, with or without PVP, for extracting high-quality RNA from mango tissues. These findings are valuable for researchers working with phenolic-rich crops, enabling reliable RNA isolation for molecular and transcriptomic analyses.