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<p><i>Averrhoa carambola L</i> is a minor fruit that has great potential to be explored at a commercial scale. It is generally propagated through seed and other vegetative propagation methods, but in vitro regeneration methods facilitate rapid regeneration of desired superior genotypes. The in vitro culture studies were conducted in carambola using nodal explants. The nodal explants were disinfected with Sodium hypochlorite (NaOCl) at 4% and Mercuric chloride (HgCl<sub>2</sub>) at 0.2–0.4% in various combinations and durations. The treatment with HgCl<sub>2</sub> (0.2%) for 5&#xa0;min followed by NaOCl (4%) for 10&#xa0;min was standardized for explant sterilization. The highest callus induction of 82.02% was achieved better in the Murashige and Skoog (MS) medium provided with 2,4-Dichlorophenoxyacetic acid (0.4&#xa0;mg/L) was very potent in callus induction and produced the best results along with a combination of BAP (6-benzylaminopurine) 4.0&#xa0;mg/L. For shoot organogenesis, the obtained results clearly state BAP 0.5&#xa0;mg/L and NAA 0.1&#xa0;mg/L (1-Napthaleneacetic Acid) at optimum concentration and combination play a major role in inducing shoot organogenesis in nodal explants of carambola.</p>

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Optimization of Sterilization, Callogenesis and Shoot Organogenesis in Carambola (Averrhoa carambola L.) Using Nodal Explants

  • 期刊论文

  • 发表日期：

    2025年5月5日

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  • J. Adarsh Reddy,
  • Prashant Kisan Nimbolkar,
  • Siddhartha Singh,
  • Amit Kumar Singh,
  • Tasso Yatung,
  • Barun Singh

摘要

Averrhoa carambola L is a minor fruit that has great potential to be explored at a commercial scale. It is generally propagated through seed and other vegetative propagation methods, but in vitro regeneration methods facilitate rapid regeneration of desired superior genotypes. The in vitro culture studies were conducted in carambola using nodal explants. The nodal explants were disinfected with Sodium hypochlorite (NaOCl) at 4% and Mercuric chloride (HgCl2) at 0.2–0.4% in various combinations and durations. The treatment with HgCl2 (0.2%) for 5 min followed by NaOCl (4%) for 10 min was standardized for explant sterilization. The highest callus induction of 82.02% was achieved better in the Murashige and Skoog (MS) medium provided with 2,4-Dichlorophenoxyacetic acid (0.4 mg/L) was very potent in callus induction and produced the best results along with a combination of BAP (6-benzylaminopurine) 4.0 mg/L. For shoot organogenesis, the obtained results clearly state BAP 0.5 mg/L and NAA 0.1 mg/L (1-Napthaleneacetic Acid) at optimum concentration and combination play a major role in inducing shoot organogenesis in nodal explants of carambola.