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Purpose <p>Chronic diabetic wounds remain difficult to treat owing to persistent inflammation and impaired tissue regeneration. This study aimed to develop a dual-functional electrospun alginate/polycaprolactone (PCL) composite dressing capable of coordinating antibacterial and regenerative actions through rational formulation design.</p> Methods <p>Silver nanoparticles were incorporated into the PCL phase to provide antibacterial activity, while the platelet-derived growth factor-B (PDGF-B) plasmid was immobilized on the alginate surface for localized gene activation. The alginate-to-PCL ratio was systematically adjusted to tune hydrophilicity, mechanical compliance, and gene-loading capacity.</p> Results <p>The optimized A8P2 formulation exhibited effective antibacterial activity and promoted in situ gene transfection, PDGF-B secretion, and in vitro fibroblast proliferation. In diabetic mice, this dressing accelerated wound closure and collagen deposition, leading to organized epithelial regeneration and reduced inflammatory response.</p> Conclusion <p>The composite dressing demonstrated biphasic therapeutic action—early antibacterial protection followed by PDGF-B–associated tissue regeneration—highlighting a compositionally tunable and spatiotemporally responsive wound-dressing platform for localized treatment of chronic diabetic wounds.</p>

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Dual-functional alginate/polycaprolactone composite dressing for co-delivery of silver nanoparticles and PDGF-B plasmid to promote diabetic wound regeneration

  • 期刊论文

  • 发表日期：

    2026年1月30日

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  • I-Tsu Chyuan,
  • Ping-Jyun Li,
  • Chia-Wen Tsao,
  • Wei-Wen Hu

摘要

Purpose

Chronic diabetic wounds remain difficult to treat owing to persistent inflammation and impaired tissue regeneration. This study aimed to develop a dual-functional electrospun alginate/polycaprolactone (PCL) composite dressing capable of coordinating antibacterial and regenerative actions through rational formulation design.

Methods

Silver nanoparticles were incorporated into the PCL phase to provide antibacterial activity, while the platelet-derived growth factor-B (PDGF-B) plasmid was immobilized on the alginate surface for localized gene activation. The alginate-to-PCL ratio was systematically adjusted to tune hydrophilicity, mechanical compliance, and gene-loading capacity.

Results

The optimized A8P2 formulation exhibited effective antibacterial activity and promoted in situ gene transfection, PDGF-B secretion, and in vitro fibroblast proliferation. In diabetic mice, this dressing accelerated wound closure and collagen deposition, leading to organized epithelial regeneration and reduced inflammatory response.

Conclusion

The composite dressing demonstrated biphasic therapeutic action—early antibacterial protection followed by PDGF-B–associated tissue regeneration—highlighting a compositionally tunable and spatiotemporally responsive wound-dressing platform for localized treatment of chronic diabetic wounds.