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<p>Drought stress significantly impacts sorghum plant metabolism, growth and quality. This study evaluated the physiological and molecular responses of <i>Sorghum bicolor</i> (L. Moench) genotypes under water deficit, with emphasis on nitrogen metabolism, forage quality, and gene expression. Two brown midrib (CSV 43, SPV 2017) and two non-brown midrib (CSV 15, SPV 462) genotypes were cultivated under field conditions and subjected to drought stress. Morpho-physiological traits, activities of key nitrogen metabolizing enzymes, along with fodder quality parameters were recorded at 45, 55, 65, and 75 days after sowing. Gene expression of <i>GLY-1</i>, <i>D-LDH</i>, <i>PAL</i>, <i>Rubisco</i>, and <i>Stag</i> was assessed in polyethylene glycol (PEG)-primed seedlings of CSV 43 and CSV 15 at 10 and 20 days after treatment. Drought stress reduced chlorophyll content, enzyme activities, and fodder quality, with more pronounced effects observed in non-BMR genotypes. BMR genotypes exhibited superior nitrogen assimilation, greater biomass accumulation, and reduced concentrations of anti-nutritional compounds. Upregulation of <i>GLY-1</i>, <i>D-LDH</i>, <i>Rubisco</i>, and <i>Stag</i> was observed in CSV 43, while <i>PAL</i> expression was higher in CSV 15. Significant associations were identified between nitrogen metabolism traits, yield and quality parameters. BMR genotypes demonstrated enhanced drought tolerance and superior forage potential, underscoring their suitability for cultivation in water-limited regions. Nitrogen metabolism traits and stress-responsive gene expression may serve as reliable selection markers for drought resilience in sorghum.</p>

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Drought-Driven Alterations in Nitrogen Metabolism and Gene Expression in Brown Midrib Sorghum

  • 期刊论文

  • 发表日期：

    2026年6月1日

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  • G. Kaur,
  • H. K. Oberoi,
  • P. Manchanda,
  • M. Kaur,
  • A. V. Umakanth

摘要

Drought stress significantly impacts sorghum plant metabolism, growth and quality. This study evaluated the physiological and molecular responses of Sorghum bicolor (L. Moench) genotypes under water deficit, with emphasis on nitrogen metabolism, forage quality, and gene expression. Two brown midrib (CSV 43, SPV 2017) and two non-brown midrib (CSV 15, SPV 462) genotypes were cultivated under field conditions and subjected to drought stress. Morpho-physiological traits, activities of key nitrogen metabolizing enzymes, along with fodder quality parameters were recorded at 45, 55, 65, and 75 days after sowing. Gene expression of GLY-1, D-LDH, PAL, Rubisco, and Stag was assessed in polyethylene glycol (PEG)-primed seedlings of CSV 43 and CSV 15 at 10 and 20 days after treatment. Drought stress reduced chlorophyll content, enzyme activities, and fodder quality, with more pronounced effects observed in non-BMR genotypes. BMR genotypes exhibited superior nitrogen assimilation, greater biomass accumulation, and reduced concentrations of anti-nutritional compounds. Upregulation of GLY-1, D-LDH, Rubisco, and Stag was observed in CSV 43, while PAL expression was higher in CSV 15. Significant associations were identified between nitrogen metabolism traits, yield and quality parameters. BMR genotypes demonstrated enhanced drought tolerance and superior forage potential, underscoring their suitability for cultivation in water-limited regions. Nitrogen metabolism traits and stress-responsive gene expression may serve as reliable selection markers for drought resilience in sorghum.