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<p>The research deals with cytological investigation on pre and post-fertilization barriers in <i>Cajanus cajan</i> (Pigeonpea variety CO 8) and wild species <i>C. cajanifolius</i> and the pollen fertility assessment and in-vitro pollen germination in <i>Cajanus scarabaeoides</i>. Pollen-pistil interaction in wide crosses could be analysed by aniline blue technique. At 2 HAP and 24 HAP. The higher and lower pollen germination percentage was found in direct and reciprocal crosses of two pigeonpea species <i>C. cajanifolius</i> and <i>Cajanus scarabaeoides</i> respectively. In direct and reciprocal cross, mean pollen germination percentage was 73.6 and 60.4% respectively. In direct cross, pod set percent is very less, due to post-zygotic fertilization barrier. In reciprocal cross, there is no pod set which indicates pre-zygotic fertilization barrier. Acetocarmine pollen fertility test reveals that <i>Cajanus cajan</i> (CO 8) is more fertile than <i>Cajanus cajanifolius</i> and hence the former one being good pollen-producing parent in terms of fertility. The best medium for in vitro pollen germination for <i>Cajanus scarabaeoides</i> isBrewbaker and Kwack medium (20% sucrose, 100mgl<sup>− 1</sup> boric acid, 300mg<sup>− 1</sup> calcium nitrate, 200mg<sup>− 1</sup> magnesium sulphate and 100mg<sup>− 1</sup> potassium nitrate). The findings were compared and strategies to alleviate the pre- and post-fertilization barriers and revealed that chromosomes have a degree of homology between <i>C. cajanifolius</i> and <i>C. cajan</i>, which is further aided by their quick crossability and production of a fertile hybrid.</p>

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Pollen Pistil Interaction Studies of Pigeonpea Wide Crosses

  • 期刊论文

  • 发表日期：

    2026年3月5日

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  • Thanga Hemavathy Arumugam,
  • Kalaiyarasi Ramachandran,
  • Karthiba Loganathan,
  • Kavitha Shanmugam,
  • VInoth Ramu,
  • Kanimoli Mathivathana,
  • Samyuktha Santhi Madhavan

摘要

The research deals with cytological investigation on pre and post-fertilization barriers in Cajanus cajan (Pigeonpea variety CO 8) and wild species C. cajanifolius and the pollen fertility assessment and in-vitro pollen germination in Cajanus scarabaeoides. Pollen-pistil interaction in wide crosses could be analysed by aniline blue technique. At 2 HAP and 24 HAP. The higher and lower pollen germination percentage was found in direct and reciprocal crosses of two pigeonpea species C. cajanifolius and Cajanus scarabaeoides respectively. In direct and reciprocal cross, mean pollen germination percentage was 73.6 and 60.4% respectively. In direct cross, pod set percent is very less, due to post-zygotic fertilization barrier. In reciprocal cross, there is no pod set which indicates pre-zygotic fertilization barrier. Acetocarmine pollen fertility test reveals that Cajanus cajan (CO 8) is more fertile than Cajanus cajanifolius and hence the former one being good pollen-producing parent in terms of fertility. The best medium for in vitro pollen germination for Cajanus scarabaeoides isBrewbaker and Kwack medium (20% sucrose, 100mgl− 1 boric acid, 300mg− 1 calcium nitrate, 200mg− 1 magnesium sulphate and 100mg− 1 potassium nitrate). The findings were compared and strategies to alleviate the pre- and post-fertilization barriers and revealed that chromosomes have a degree of homology between C. cajanifolius and C. cajan, which is further aided by their quick crossability and production of a fertile hybrid.