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<Emphasis Type="BoldItalic">BACKGROUND:</Emphasis> <p>The contraction behaviors of cardiomyocytes (CMs), especially contraction synchrony, are crucial factors reflecting their maturity and response to drugs. A wider field of view helps to observe more pronounced synchrony differences, but the accompanied greater computational load, requiring more computing power or longer computational time.</p> <Emphasis Type="BoldItalic">METHODS:</Emphasis> <p>We proposed a method that directly correlates variations in optical field brightness with cardiac tissue contraction status (CVB method), based on principles from physics and photometry, for rapid video analysis in wide field of view to obtain contraction parameters, such as period and contraction propagation direction and speed.</p> <Emphasis Type="BoldItalic">RESULTS:</Emphasis> <p>Through video analysis of human induced pluripotent stem cell (hiPSC)-derived CMs labeled with green fluorescent protein (GFP) cultured on aligned and random nanofiber scaffolds, the CVB method was demonstrated to obtain contraction parameters and quantify the direction and speed of contraction within regions of interest (ROIs) in wide field of view. The CVB method required less computation time compared to one of the contour tracking methods, the Lucas-Kanade (LK) optical flow method, and provided better stability and accuracy in the results.</p> <Emphasis Type="BoldItalic">CONCLUSION:</Emphasis> <p>This method has a smaller computational load, is less affected by motion blur and out-of-focus conditions, and provides a potential tool for accurate and rapid analysis of cardiac tissue contraction synchrony in wide field of view without the need for more powerful hardware.</p>

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Rapid Video Analysis for Contraction Synchrony of Human Induced Pluripotent Stem Cells-Derived Cardiac Tissues

  • 期刊论文

  • 发表日期：

    2025年1月13日

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  • Yuqing Jiang,
  • Mingcheng Xue,
  • Lu Ou,
  • Huiquan Wu,
  • Jianhui Yang,
  • Wangzihan Zhang,
  • Zhuomin Zhou,
  • Qiang Gao,
  • Bin Lin,
  • Weiwei Kong,
  • Songyue Chen,
  • Daoheng Sun

摘要

BACKGROUND:

The contraction behaviors of cardiomyocytes (CMs), especially contraction synchrony, are crucial factors reflecting their maturity and response to drugs. A wider field of view helps to observe more pronounced synchrony differences, but the accompanied greater computational load, requiring more computing power or longer computational time.

METHODS:

We proposed a method that directly correlates variations in optical field brightness with cardiac tissue contraction status (CVB method), based on principles from physics and photometry, for rapid video analysis in wide field of view to obtain contraction parameters, such as period and contraction propagation direction and speed.

RESULTS:

Through video analysis of human induced pluripotent stem cell (hiPSC)-derived CMs labeled with green fluorescent protein (GFP) cultured on aligned and random nanofiber scaffolds, the CVB method was demonstrated to obtain contraction parameters and quantify the direction and speed of contraction within regions of interest (ROIs) in wide field of view. The CVB method required less computation time compared to one of the contour tracking methods, the Lucas-Kanade (LK) optical flow method, and provided better stability and accuracy in the results.

CONCLUSION:

This method has a smaller computational load, is less affected by motion blur and out-of-focus conditions, and provides a potential tool for accurate and rapid analysis of cardiac tissue contraction synchrony in wide field of view without the need for more powerful hardware.