Scholarlink

<p>This study characterized the plastic degradation potential of bacterial isolates from contaminated soils of Jarma and KUST. Bacteriological analysis identified <i>Pseudomonas</i> sp. (J1) as the dominant Gram-negative isolate, exhibiting robust growth (OD<sub>600</sub> = 0.454, 8.2 × 10<sup>8</sup>&#xa0;CFU/ml) in minimal salt media with µmax = 0.12&#xa0;h<sup>−1</sup>, while <i>Bacillus</i> sp. (K1) showed biphasic growth (OD<sub>600</sub> = 0.219 at day 9). <i>Pseudomonas</i> sp. demonstrated superior degradation of canteen store department (CSD) plastic with 10.12% weight loss (<i>p</i> &lt; 0.001 vs <i>Bacillus</i>’s 8.47%), forming extensive biofilms (&gt; 85% coverage) and causing significant surface alterations visible in scanning electron microscopy (SEM). Fourier transform infrared spectroscopy (FTIR) analysis revealed oxidative degradation markers (new O–H peak at 3367.98&#xa0;cm<sup>−1</sup> and C = C bond formation at 1648.31&#xa0;cm<sup>−1</sup>), with <i>Pseudomonas</i> showing more pronounced structural modifications. Molecular characterization confirmed both isolates harbored PETase genes (465&#xa0;bp), and 16S rRNA phylogeny identified them as <i>Pseudomonas aeruginosa</i> and <i>Bacillus subtilis</i>. These results highlight <i>Pseudomonas</i> sp.’s enhanced plastic degradation efficiency, particularly on starch-based polymers (CSD plastic), attributed to its superior biofilm formation (µmax = 0.15&#xa0;h<sup>−1</sup>) and enzymatic activity. The findings suggest these isolates hold promise for bioremediation applications, though further optimization of culture conditions and enzyme profiling is needed to enhance degradation rates for practical implementation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Evaluation of plastic degradation potential of soil bacteria from waste sites: structural and functional characterization

  • 期刊论文

  • 发表日期：

    2026年4月27日

查看全文
  • M. Khan,
  • A. Rehman,
  • M. Anees,
  • M. S. Al Tami,
  • H. Naveed,
  • M. Hussain,
  • I. Naz

摘要

This study characterized the plastic degradation potential of bacterial isolates from contaminated soils of Jarma and KUST. Bacteriological analysis identified Pseudomonas sp. (J1) as the dominant Gram-negative isolate, exhibiting robust growth (OD600 = 0.454, 8.2 × 108 CFU/ml) in minimal salt media with µmax = 0.12 h−1, while Bacillus sp. (K1) showed biphasic growth (OD600 = 0.219 at day 9). Pseudomonas sp. demonstrated superior degradation of canteen store department (CSD) plastic with 10.12% weight loss (p < 0.001 vs Bacillus’s 8.47%), forming extensive biofilms (> 85% coverage) and causing significant surface alterations visible in scanning electron microscopy (SEM). Fourier transform infrared spectroscopy (FTIR) analysis revealed oxidative degradation markers (new O–H peak at 3367.98 cm−1 and C = C bond formation at 1648.31 cm−1), with Pseudomonas showing more pronounced structural modifications. Molecular characterization confirmed both isolates harbored PETase genes (465 bp), and 16S rRNA phylogeny identified them as Pseudomonas aeruginosa and Bacillus subtilis. These results highlight Pseudomonas sp.’s enhanced plastic degradation efficiency, particularly on starch-based polymers (CSD plastic), attributed to its superior biofilm formation (µmax = 0.15 h−1) and enzymatic activity. The findings suggest these isolates hold promise for bioremediation applications, though further optimization of culture conditions and enzyme profiling is needed to enhance degradation rates for practical implementation.