<p>Bioremediation is an eco-friendly strategy to mitigate soil pollution by leveraging the natural capabilities of plants. In this study, we explored the potential of <i>Cereus jamacaru</i> (Mandacaru), a cactus native to the Brazilian Caatinga, for bioremediating environments contaminated with heavy metals. Using bioinformatics and molecular docking techniques, we identified proteins with the potential to interact with heavy metals, based on transcriptomic data. The analysis revealed promising protein–metal interaction patterns, indicating the potential for remediation of multiple heavy metals in contaminated environments. Additionally, our study uncovered physiological mechanisms and metabolic pathways affected by the presence of these metals, offering insights into mandacaru's adaptive strategies. Our results highlight proteins that interact with heavy metals in silico, although experimental validation is necessary to confirm these findings. Thus, this study contributes not only to the understanding of mandacaru biology but also to the development of bioremediation strategies in the field of environmental biotechnology.</p>