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<p>The petroleum hydrocarbon pollution of the environment poses serious ecological problems and affects human health negatively. The development of an efficient, environmentally friendly, and cost-effective method to remediate petroleum hydrocarbon-polluted areas is therefore imperative. This study isolated hydrocarbon-degrading bacteria and fungi from the soils of bitumen-polluted areas of Agbabu in Ondo State, Southwestern Nigeria. The isolates were screened for their relative bitumen degradative activities based on their growth on a medium, with 1.0% w/v bitumen as the sole carbon source, using optical density measurement. The influence of three different bitumen concentrations 1.0%, 1.5%, and 2.0% w/v, on degradation activity, was also determined. Five bacterial strains with the most appreciative bitumen degradation ability were characterized and identified by phenotypic and molecular methods as <i>Brevundimonas naejangsaniensis</i> A1, <i>Chryseobacterium gleum</i> C1, <i>Chryseobacterium gleum</i> Z2, <i>Enterobacter mori</i> D3, and <i>Enterobacter mori</i> E1. Four fungal strains, observed to exhibit the most appreciable bitumen degradation potential were two <i>Aspergillus</i> spp., <i>Sclerotium</i> sp., and <i>Flagellospora</i> sp. The bacterial species presented a much better bitumen degradative potential than the fungal species. The lowest bitumen concentration (1.0% w/v) favoured the rapid growth of the study organisms. While the organisms had a slower growth rate initially for the higher bitumen concentrations, their activities increased steadily with increase in incubation periods. The bacterial species isolated from bitumen polluted soils in Agbabu, Ondo State in Southwestern Nigeria demonstrated appreciable bitumen degradation capability and could be optimized for use in the bioremediation of bitumen and other petroleum hydrocarbon-polluted areas.</p>

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Isolation, characterization, and hydrocarbon-degrading potentials of microorganisms from bitumen-polluted soils of Agbabu, Ondo State, Nigeria

  • 期刊论文

  • 发表日期：

    2025年6月17日

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  • A. A. Adewusi,
  • O. Oyedeji,
  • S. M. Adeyemo,
  • O. O. Awotoye

摘要

The petroleum hydrocarbon pollution of the environment poses serious ecological problems and affects human health negatively. The development of an efficient, environmentally friendly, and cost-effective method to remediate petroleum hydrocarbon-polluted areas is therefore imperative. This study isolated hydrocarbon-degrading bacteria and fungi from the soils of bitumen-polluted areas of Agbabu in Ondo State, Southwestern Nigeria. The isolates were screened for their relative bitumen degradative activities based on their growth on a medium, with 1.0% w/v bitumen as the sole carbon source, using optical density measurement. The influence of three different bitumen concentrations 1.0%, 1.5%, and 2.0% w/v, on degradation activity, was also determined. Five bacterial strains with the most appreciative bitumen degradation ability were characterized and identified by phenotypic and molecular methods as Brevundimonas naejangsaniensis A1, Chryseobacterium gleum C1, Chryseobacterium gleum Z2, Enterobacter mori D3, and Enterobacter mori E1. Four fungal strains, observed to exhibit the most appreciable bitumen degradation potential were two Aspergillus spp., Sclerotium sp., and Flagellospora sp. The bacterial species presented a much better bitumen degradative potential than the fungal species. The lowest bitumen concentration (1.0% w/v) favoured the rapid growth of the study organisms. While the organisms had a slower growth rate initially for the higher bitumen concentrations, their activities increased steadily with increase in incubation periods. The bacterial species isolated from bitumen polluted soils in Agbabu, Ondo State in Southwestern Nigeria demonstrated appreciable bitumen degradation capability and could be optimized for use in the bioremediation of bitumen and other petroleum hydrocarbon-polluted areas.