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<p>The Jajrood River serves as a crucial drinking water source for Tehran, Iran, necessitating regular monitoring of its pollution levels to safeguard public health. Endotoxin remains a significant pollutant of concern, adversely affecting water quality. This study evaluates two detection methods for endotoxin levels: traditional Limulus Amebocyte Lysate testing and a bioluminescence inhibition assay using <i>Vibrio fischeri</i>. The objective is to compare the efficiency and accuracy of these methods in assessing water quality in both the Jajrood River and a connected water treatment facility. The findings reveal that the <i>Vibrio fischeri</i> assay can detect endotoxin levels as low as &lt; 1 EU/mL within 15&#xa0;min, significantly faster than the 120&#xa0;min required for Limulus Amebocyte Lysate testing. However, external environmental factors impacted the accuracy of the <i>Vibrio fischeri</i> results. Notably, strong correlations were observed between the two methods (r = 0.61 for the river; r = 0.46 for the treatment plant), underscoring the urgent need for enhanced monitoring strategies for drinking water in Tehran. This research highlights the potential of innovative detection methods to improve public health safety.</p>

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Comparative assessment of endotoxin levels in drinking water: bioluminescence inhibition and Limulus Amebocyte Lysate (LAL) testing

  • 期刊论文

  • 发表日期：

    2025年3月11日

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  • R. Dehghan,
  • Kh. Piri,
  • A. Abdoli

摘要

The Jajrood River serves as a crucial drinking water source for Tehran, Iran, necessitating regular monitoring of its pollution levels to safeguard public health. Endotoxin remains a significant pollutant of concern, adversely affecting water quality. This study evaluates two detection methods for endotoxin levels: traditional Limulus Amebocyte Lysate testing and a bioluminescence inhibition assay using Vibrio fischeri. The objective is to compare the efficiency and accuracy of these methods in assessing water quality in both the Jajrood River and a connected water treatment facility. The findings reveal that the Vibrio fischeri assay can detect endotoxin levels as low as < 1 EU/mL within 15 min, significantly faster than the 120 min required for Limulus Amebocyte Lysate testing. However, external environmental factors impacted the accuracy of the Vibrio fischeri results. Notably, strong correlations were observed between the two methods (r = 0.61 for the river; r = 0.46 for the treatment plant), underscoring the urgent need for enhanced monitoring strategies for drinking water in Tehran. This research highlights the potential of innovative detection methods to improve public health safety.