Designing of Phosphate Rich Compost Inoculated with Environmental Strain Pseudomonas aeruginosa AAC1 As A Phosphate Solubilizer
摘要
In the present study, Pseudomonas aeruginosa AAC1 was utilized to design a phosphorus-rich compost. Physicochemical and Illumina MiSeq sequencing were employed to explore the effect of inoculation on the microbial diversity in inoculated pile, which was compared with an un-inoculated compost pile. The findings indicate that compost maturity was achieved by 60th day, characterized by optimal temperature (28 °C), pH (7.1—7.5), and moisture content (32% – 38%). The relative abundance of P. aeruginosa decreased in mature compost, coinciding with a notable increase in the available phosphorus level, reaching up to 6.82 g kg−1 in inoculated compost piles. The addition of P. aeruginosa AAC1 did not influence the C, N, C: N ratio, organic matter, and ash content but significantly decreased the pH and moisture contents and increased the composting temperature, available phosphorus, calcium, and magnesium content. The inoculation of Pseudomonas aeruginosa AAC1 in compost did not significantly alter the structure of the microbial community, since metagenomic analysis revealed similar microbial communities in inoculated and un-inoculated compost piles. Overall, the dominated bacterial genera were Actinobacteria, Proteobacteria, Bacteroidetes, and Firmicutes at the phylum level whereas, the fungal communities were densely populated with Ascomycota, Aphelidiomycota, Chytridiomycota, and Cercozoa. This study confirmed the potential of Pseudomonas aeruginosa AAC1 as a phosphatic bioinoculant by ameliorating the physiochemical conditions of compost without compromising the microbial diversity of compost.