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<p><i>Sitobion miscanthi</i> is a&#xa0;wheat aphid&#xa0;species that can damage seriously agricultural production. The effective management of wheat aphids has depended on chemical insecticides. However, their wide application led to severe resistance of wheat aphids to some insecticides, and cytochrome P450 as a detoxifying enzyme plays a crucial role in the insecticide resistance. In this study, <i>CYP6CY1</i>, a new P450 gene, was isolated and overexpressed in a <i>S.miscanthi</i> resistant strain to imidacloprid. The increased sensitivity to imidacloprid after silencing of <i>CYP6CY1</i> indicated that its involvement in imidacloprid resistance. Subsequently, action of&#xa0;miR-3047-3p&#xa0;within the 3′ UTR of the&#xa0;<i>CYP6CY1</i> was confirmed at the posttranscriptional regulatory level, and was shown to be&#xa0;involved in the resistance to imidacloprid. These results provide opportunities for understanding the roles of P450 in insecticide resistance of pests.</p>

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Overexpression of CYP6CY1 is Involved in Imidacloprid Resistance in Sitobion miscanthi (Takahashi) (Homoptera: Aphidae)

  • 期刊论文

  • 发表日期：

    2025年1月14日

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  • Yun-Shan Xiong,
  • Ling-Ling Cui,
  • Gui-Lei Hu,
  • Yu-Tai Jiang,
  • Yong-Po Lv,
  • Pei Zhang,
  • Jin-Song Zheng,
  • Bai-Zhong Zhang,
  • Run-Qiang Liu

摘要

Sitobion miscanthi is a wheat aphid species that can damage seriously agricultural production. The effective management of wheat aphids has depended on chemical insecticides. However, their wide application led to severe resistance of wheat aphids to some insecticides, and cytochrome P450 as a detoxifying enzyme plays a crucial role in the insecticide resistance. In this study, CYP6CY1, a new P450 gene, was isolated and overexpressed in a S.miscanthi resistant strain to imidacloprid. The increased sensitivity to imidacloprid after silencing of CYP6CY1 indicated that its involvement in imidacloprid resistance. Subsequently, action of miR-3047-3p within the 3′ UTR of the CYP6CY1 was confirmed at the posttranscriptional regulatory level, and was shown to be involved in the resistance to imidacloprid. These results provide opportunities for understanding the roles of P450 in insecticide resistance of pests.