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<p><?tk 2?>A new technique of boron determination by graphite furnace atomic absorption spectrometry (GFAAS) in animal tissues after administration in nanoparticle form has been developed and validated. The applicability of an existing GFAAS method for manganese determination in similar biological samples was also verified, and its analytical characteristics were evaluated. GFAAS determination was verified by spike experiments and by independent measurements by inductively coupled plasma optical emission spectrometry. The F- and t-tests of the obtained values were calculated; the recovery values were not less than 80%. The effect of various chemical modifiers on the analytical signal was studied. The Zr-Ni modifier was found to reduce boron losses through volatile molecular compound formation at the pyrolysis stage, lower the atomization temperature, and enable reliable boron quantification. The developed technique enables determination of boron and manganese in samples from both in vivo and in vitro experiments. Under optimized conditions, the lowest achievable limits of quantification in animal tissues were found to be (µg g<sup>–1</sup>) 0.7–3.5 and 0.5 × 10<sup>–3</sup> – 1.1 × 10<sup>–2</sup> for boron and manganese, respectively; in cells – 1.1 × 10<sup>–4</sup> µg for boron, 3.2 × 10<sup>–7</sup> µg for manganese. The good applicability and green status of the proposed technique were confirmed using the analytical eco-scale, the green analytical procedure index and the blue applicability grade index.</p>

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GFAAS determination of boron and manganese in biological samples after their administration by nanoparticles form

  • 期刊论文

  • 发表日期：

    2026年7月19日

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  • T. Ya. Guselnikova,
  • A. V. Volzhenin,
  • Y. V. Lepeshonkova,
  • A. R. Tsygankova,
  • S. A. Uspenskii,
  • O. I. Kichakova

摘要

A new technique of boron determination by graphite furnace atomic absorption spectrometry (GFAAS) in animal tissues after administration in nanoparticle form has been developed and validated. The applicability of an existing GFAAS method for manganese determination in similar biological samples was also verified, and its analytical characteristics were evaluated. GFAAS determination was verified by spike experiments and by independent measurements by inductively coupled plasma optical emission spectrometry. The F- and t-tests of the obtained values were calculated; the recovery values were not less than 80%. The effect of various chemical modifiers on the analytical signal was studied. The Zr-Ni modifier was found to reduce boron losses through volatile molecular compound formation at the pyrolysis stage, lower the atomization temperature, and enable reliable boron quantification. The developed technique enables determination of boron and manganese in samples from both in vivo and in vitro experiments. Under optimized conditions, the lowest achievable limits of quantification in animal tissues were found to be (µg g–1) 0.7–3.5 and 0.5 × 10–3 – 1.1 × 10–2 for boron and manganese, respectively; in cells – 1.1 × 10–4 µg for boron, 3.2 × 10–7 µg for manganese. The good applicability and green status of the proposed technique were confirmed using the analytical eco-scale, the green analytical procedure index and the blue applicability grade index.