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<p>In recent years, Au nanomaterials-based artificial peroxidase has been widely used for the determination of glucose due to their specific properties. Santa Barbara Amorphous-15 containing Au nanoparticles (Au-SBA-15) were prepared and discovered to function like peroxidase in this study. The peroxidase mimic activity of Au-SBA-15 was investigated using 3,3’,5,5’-tetramethylbenzidine (TMB) as a chromogen. The best catalytic activity was obtained at 40℃ and pH = 3.0. The color change and absorbance intensity are dependent on the concentration of H<sub>2</sub>O<sub>2</sub>, thus the direct determination of H<sub>2</sub>O<sub>2</sub> and indirect detection of glucose were demonstrated using Au-SBA-15 with a limit of detections (LOD) of 0.4µM and 2.8µM, respectively. In addition, present method was applied for detection of glucose in 60% serum with a LOD of 11.4µM. Considering the excellent peroxidase mimic activity, Au-SBA-15 is expected to have potential applications in colorimetric detection of various substances.</p>

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Colorimetric sensing of glucose based on Au-SBA-15 with peroxidase mimic activity

  • 期刊论文

  • 发表日期：

    2026年5月1日

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  • Mi-Gyong Pak,
  • Kwang-Chon Pae,
  • Chol-San Jon,
  • Yun-Hui Ju,
  • Yong-Gil Pak,
  • Kum-Chol Ri

摘要

In recent years, Au nanomaterials-based artificial peroxidase has been widely used for the determination of glucose due to their specific properties. Santa Barbara Amorphous-15 containing Au nanoparticles (Au-SBA-15) were prepared and discovered to function like peroxidase in this study. The peroxidase mimic activity of Au-SBA-15 was investigated using 3,3’,5,5’-tetramethylbenzidine (TMB) as a chromogen. The best catalytic activity was obtained at 40℃ and pH = 3.0. The color change and absorbance intensity are dependent on the concentration of H2O2, thus the direct determination of H2O2 and indirect detection of glucose were demonstrated using Au-SBA-15 with a limit of detections (LOD) of 0.4µM and 2.8µM, respectively. In addition, present method was applied for detection of glucose in 60% serum with a LOD of 11.4µM. Considering the excellent peroxidase mimic activity, Au-SBA-15 is expected to have potential applications in colorimetric detection of various substances.