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<p>Water-soluble vitamins in nutraceutical powder formulations are highly prone to degradation during processing and storage, yet Their stability is inadequately monitored by existing analytical methods. To address this critical gap, High-Performance Thin-Layer Chromatography (HPTLC) was employed as a novel, reliable technique to accurately track vitamin loss and enhance quality assurance in nutraceutical products. A High-Performance Thin Layer Chromatography technique was employed to evaluate and quantify Thiamine in both nutraceutical tablet and syrup formulations. Throughout the optimization stage of the procedure, TLC Silica gel 60 F254 plates were identified as a suitable stationary phase, while a uniform solution comprising Ethanol: Acetic acid: Chloroform: Liquor ammonia: Water (5.5:2.5:4.5:2:1 v/v/v/v/v) was utilised as mobile phase. The thiamine displayed a prominent peak at a wavelength of 254&#xa0;nm, which was the specific wavelength at which the analysis was conducted. The method’s validation conformed to the standards set forth by the International Conference on Harmonisation (ICH). The correlation coefficient was ascertained to be 0.9957, with the percentage relative standard deviation (% RSD) fluctuating between 0.3014 and 0.8511%, thereby indicating satisfactory precision. The limits of detection and quantification were determined to be 8.82&#xa0;µg and 29.41&#xa0;µg per band, respectively. The proficient quantitative evaluation of thiamine across diverse nutraceutical formulations highlighted the effectiveness of the validated method for quality control, which also demonstrates greater environmental sustainability than alternative analytical approaches, as it necessitates minimal usage of solvents and reagents.</p>

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Selective analysis of thiamine content in dietary formulations using HPTLC

  • 期刊论文

  • 发表日期：

    2026年2月16日

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  • Apurva Wasnik,
  • Vaishnavi Chaudhari,
  • Krishna R. Gupta,
  • Milind J. Umekar

摘要

Water-soluble vitamins in nutraceutical powder formulations are highly prone to degradation during processing and storage, yet Their stability is inadequately monitored by existing analytical methods. To address this critical gap, High-Performance Thin-Layer Chromatography (HPTLC) was employed as a novel, reliable technique to accurately track vitamin loss and enhance quality assurance in nutraceutical products. A High-Performance Thin Layer Chromatography technique was employed to evaluate and quantify Thiamine in both nutraceutical tablet and syrup formulations. Throughout the optimization stage of the procedure, TLC Silica gel 60 F254 plates were identified as a suitable stationary phase, while a uniform solution comprising Ethanol: Acetic acid: Chloroform: Liquor ammonia: Water (5.5:2.5:4.5:2:1 v/v/v/v/v) was utilised as mobile phase. The thiamine displayed a prominent peak at a wavelength of 254 nm, which was the specific wavelength at which the analysis was conducted. The method’s validation conformed to the standards set forth by the International Conference on Harmonisation (ICH). The correlation coefficient was ascertained to be 0.9957, with the percentage relative standard deviation (% RSD) fluctuating between 0.3014 and 0.8511%, thereby indicating satisfactory precision. The limits of detection and quantification were determined to be 8.82 µg and 29.41 µg per band, respectively. The proficient quantitative evaluation of thiamine across diverse nutraceutical formulations highlighted the effectiveness of the validated method for quality control, which also demonstrates greater environmental sustainability than alternative analytical approaches, as it necessitates minimal usage of solvents and reagents.