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<p>Research has extensively explored the interaction of small molecules with specific DNA regions. Spectroscopic methods were used to study the binding of salicylic hydroxamic acid (SHA) and fuchsine basic (FB) to DNA. The affinity of these dyes for CTDNA was further analyzed using different spectroscopic techniques, such as docking studies, UV–Vis, and CD spectroscopy. FB demonstrated binding affinities of (4.7 ± 0.4) × 10<sup>4</sup>&#xa0;L&#xa0;mol<sup>−1</sup> by intercalating with CTDNA. FB binds to CTDNA through non-covalent interaction which plays a crucial role in achieving the binding affinity. Circular dichroism spectroscopy results suggest that FB interaction with DNA does not lead to significant structural alterations. By contrast, SHA's lack of interaction with DNA might be attributed to a poor fit within the DNA binding pocket.</p>

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Elucidating the DNA binder interaction: a spectroscopic and computational study of fuchsine basic and salicylic hydroxamic acid

  • 期刊论文

  • 发表日期：

    2025年5月12日

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  • Ammar M. K. AL-Azzawi,
  • Ekhlas Abdallah Hassan,
  • Wassan Baqir Ali

摘要

Research has extensively explored the interaction of small molecules with specific DNA regions. Spectroscopic methods were used to study the binding of salicylic hydroxamic acid (SHA) and fuchsine basic (FB) to DNA. The affinity of these dyes for CTDNA was further analyzed using different spectroscopic techniques, such as docking studies, UV–Vis, and CD spectroscopy. FB demonstrated binding affinities of (4.7 ± 0.4) × 104 L mol−1 by intercalating with CTDNA. FB binds to CTDNA through non-covalent interaction which plays a crucial role in achieving the binding affinity. Circular dichroism spectroscopy results suggest that FB interaction with DNA does not lead to significant structural alterations. By contrast, SHA's lack of interaction with DNA might be attributed to a poor fit within the DNA binding pocket.