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<p>A simple, quick, and accurate derivative spectrophotometric technique was created to determine the individual and simultaneous presence of doxycycline hyclate (DOXH) in veterinary samples. The derivative spectra were analyzed by calculating the derivative values using graphical techniques, which included peak-to-baseline, peak-to-peak, and area under peak. The best relationships between DOXH concentrations and peak amplitudes were observed at 219&#xa0;nm and 232&#xa0;nm for 2nd derivative and 226, 239, 267, and 281&#xa0;nm for 3rd derivative, over the concentration range of 1.0–46&#xa0;μg/ml for 2nd derivative then 1.0–46&#xa0;μg/ml for 226, 281&#xa0;nm and 2.0–46&#xa0;μg/ml for 239, 267&#xa0;nm for 3rd derivative. To determine the linear relationship between concentrations and area under peaks, the peak-to-peak area method was applied at certain wavelength intervals for 2nd and 3rd derivatives. The peak area is measured within the wavelength intervals of 213–224&#xa0;nm and 224–242&#xa0;nm for the second-order derivative spectra. For the third-order derivative spectra, the peak areas are measured within the following wavelength intervals: 218–233&#xa0;nm, 233–248&#xa0;nm, 258–274&#xa0;nm, and 274–292&#xa0;nm. It was found that the methods worked well enough, with linearity and LOD values lower than 0.624 and 0.660&#xa0;μg/mL for figuring out the second- and third-order derivatives of DOXH and 0.287 and 0.951&#xa0;μg/mL for first-order derivatives simultaneous determination of florfenicol (FF) and DOXH, respectively. The suggested approach was used to determine the amount of doxycycline hyclate in pharmaceutical tablets with a recovery rate of 96.8% to 99.6% with an RSD less than 4.96%. The individual and simultaneous quantification of DOXH in veterinary samples can be accomplished with remarkable effectiveness by using the suggested approaches.</p>

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Individual and simultaneous determination of doxycycline hyclate in veterinary sample

  • 期刊论文

  • 发表日期：

    2025年4月8日

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  • Hawbir Muhammed Khdir,
  • Bnar Mahmoud Ibrahim

摘要

A simple, quick, and accurate derivative spectrophotometric technique was created to determine the individual and simultaneous presence of doxycycline hyclate (DOXH) in veterinary samples. The derivative spectra were analyzed by calculating the derivative values using graphical techniques, which included peak-to-baseline, peak-to-peak, and area under peak. The best relationships between DOXH concentrations and peak amplitudes were observed at 219 nm and 232 nm for 2nd derivative and 226, 239, 267, and 281 nm for 3rd derivative, over the concentration range of 1.0–46 μg/ml for 2nd derivative then 1.0–46 μg/ml for 226, 281 nm and 2.0–46 μg/ml for 239, 267 nm for 3rd derivative. To determine the linear relationship between concentrations and area under peaks, the peak-to-peak area method was applied at certain wavelength intervals for 2nd and 3rd derivatives. The peak area is measured within the wavelength intervals of 213–224 nm and 224–242 nm for the second-order derivative spectra. For the third-order derivative spectra, the peak areas are measured within the following wavelength intervals: 218–233 nm, 233–248 nm, 258–274 nm, and 274–292 nm. It was found that the methods worked well enough, with linearity and LOD values lower than 0.624 and 0.660 μg/mL for figuring out the second- and third-order derivatives of DOXH and 0.287 and 0.951 μg/mL for first-order derivatives simultaneous determination of florfenicol (FF) and DOXH, respectively. The suggested approach was used to determine the amount of doxycycline hyclate in pharmaceutical tablets with a recovery rate of 96.8% to 99.6% with an RSD less than 4.96%. The individual and simultaneous quantification of DOXH in veterinary samples can be accomplished with remarkable effectiveness by using the suggested approaches.