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<p>Medicinal plants or herbs act as reservoirs of therapeutic agents and become source of traditional or conventional treatment of various health issues. <i>Enhydra fluctuans</i> Lour. is one such plant belongs to Asteraceae family, mostly using traditionally as vegetable or its decoction to treat hyperglycaemia, although scientific exploration&#xa0;for this claim is very limited. In this study, we evaluated the hypoglycaemic activity of&#xa0;<i>E. fluctuans</i> Lour. along with their phytochemical screening, in-silico study and anti-oxidant activity. Phytoconstituents&#xa0;belongs to alkaloids, flavonoids, terpenoids, phenolic compounds, carbohydrates, etc. were found in preliminary&#xa0;phytochemical investigations. In-silico study has been performed on its phytoconstituents and it has been found that two compound C5 (8-Desacyl enhydrin- [4- hydroxytiglate]) and C8 (Baicalein 7-O-glucoside) bind with all three proteins <i>i.e.</i> 7K9N, 3BC9 and 5ISO with binding energy of − 188.31, − 243.57; − 169.90, − 202.13 and − 296.05, − 271.38 kcal/mol respectively as compared to standard acarbose voglibose and metformin − 152.993, 142.19 and − 215.55&#xa0;kcal/mol respectively. In-vitro antidiabetic study was performed for methanolic extract against α-glucosidase and α-amylase inhibition, where IC<sub>50</sub> value was found to be 123.79 ± 3.522 and 155.52 ± 5.890&#xa0;µg/ml as compared to standard acarbose 206. 75 ± 5.082 and 120.38 ± 3.687&#xa0;µg/ml respectively. Both the antioxidant and enzymatic inhibition study revealed that methanolic extract of <i>Enhydra fluctuans</i> have potential to reduce DPPH and hydroxyl free radical. It has also been observed that methanolic extract exhibit better&#xa0;α-glucosidase inhibition as compared to acarbose but lesser inhibition against&#xa0;α-amylase which indicate that methanolic extract might have good antidiabetic potential. Moreover, in silico study revealed that, both the compound C5 and C8 might be responsible for enzymatic inhibition. Overall findings demonstrate that <i>E. fluctuans</i> Lour. have&#xa0;significant antioxidant and antidiabetic activity. </p>

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In-vitro and in-silico analysis of phytoconstituents of Enhydra fluctuans as an antidiabetic agent

  • 期刊论文

  • 发表日期：

    2025年3月14日

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  • Shahnaz Alom,
  • Farak Ali,
  • Rajashri Bezbaruah,
  • Bibhuti Bhusan Kakoti

摘要

Medicinal plants or herbs act as reservoirs of therapeutic agents and become source of traditional or conventional treatment of various health issues. Enhydra fluctuans Lour. is one such plant belongs to Asteraceae family, mostly using traditionally as vegetable or its decoction to treat hyperglycaemia, although scientific exploration for this claim is very limited. In this study, we evaluated the hypoglycaemic activity of E. fluctuans Lour. along with their phytochemical screening, in-silico study and anti-oxidant activity. Phytoconstituents belongs to alkaloids, flavonoids, terpenoids, phenolic compounds, carbohydrates, etc. were found in preliminary phytochemical investigations. In-silico study has been performed on its phytoconstituents and it has been found that two compound C5 (8-Desacyl enhydrin- [4- hydroxytiglate]) and C8 (Baicalein 7-O-glucoside) bind with all three proteins i.e. 7K9N, 3BC9 and 5ISO with binding energy of − 188.31, − 243.57; − 169.90, − 202.13 and − 296.05, − 271.38 kcal/mol respectively as compared to standard acarbose voglibose and metformin − 152.993, 142.19 and − 215.55 kcal/mol respectively. In-vitro antidiabetic study was performed for methanolic extract against α-glucosidase and α-amylase inhibition, where IC50 value was found to be 123.79 ± 3.522 and 155.52 ± 5.890 µg/ml as compared to standard acarbose 206. 75 ± 5.082 and 120.38 ± 3.687 µg/ml respectively. Both the antioxidant and enzymatic inhibition study revealed that methanolic extract of Enhydra fluctuans have potential to reduce DPPH and hydroxyl free radical. It has also been observed that methanolic extract exhibit better α-glucosidase inhibition as compared to acarbose but lesser inhibition against α-amylase which indicate that methanolic extract might have good antidiabetic potential. Moreover, in silico study revealed that, both the compound C5 and C8 might be responsible for enzymatic inhibition. Overall findings demonstrate that E. fluctuans Lour. have significant antioxidant and antidiabetic activity.