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<p>Cucumber (<i>Cucumis sativus</i> L.) production is often compromised by drought stress, resulting in significant yield and quality losses. MicroRNAs (miRNAs) are small non-coding RNAs that play essential roles in regulating plant growth, development, and stress responses. In a previous study, our team obtained miRNA, transcriptome, and degradome sequencing data from drought-stressed cucumber seedlings. Using integrated analyses, we identified 17 members of the miR156 family (Csa-miR156s) and their corresponding target genes in the current study. Bioinformatic approaches were employed to characterize the Csa-miR156s, analyze their expression profiles, and investigate their regulatory relationships with target genes. Additionally, quantitative real-time PCR (qPCR) was used to assess the expression of Csa-miR156s under PEG-simulated drought stress. The results indicated that the miR156 family is divided into four major branches, with the 17 members distributed across them. Fifteen Csa-miR156s were located on five cucumber chromosomes, while the remaining two were repeatedly distributed in chloroplast, mitochondrial, and scaffold sequences. The 17 mature Csa-miR156s were derived from 17 distinct precursor sequences, all of which could form stable stem-loop secondary structures. Analysis of promoter regions revealed that Csa-miR156s contained <i>cis</i>-acting elements associated with responses to light, hormones, or stresses, as well as biosynthesis and metabolism. Expression analyses showed differential expression patterns between Csa-miR156s and their target genes, and qPCR confirmed significant changes in Csa-miR156 expression under drought stress compared with controls. These results suggest that Csa-miR156s are likely involved in the drought stress response in cucumber, thus providing a robust theoretical foundation and offering candidate genes for the future genetic improvement of drought tolerance in cucumber.</p>

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Genome-wide characterization of the Csa-miR156 family revels its dynamic role in drought stress response in cucumber

  • 期刊论文

  • 发表日期：

    2026年7月22日

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  • Lili Guan,
  • Lei Zhou,
  • Qian Yu,
  • Yanchang Jiang,
  • Defang Gan

摘要

Cucumber (Cucumis sativus L.) production is often compromised by drought stress, resulting in significant yield and quality losses. MicroRNAs (miRNAs) are small non-coding RNAs that play essential roles in regulating plant growth, development, and stress responses. In a previous study, our team obtained miRNA, transcriptome, and degradome sequencing data from drought-stressed cucumber seedlings. Using integrated analyses, we identified 17 members of the miR156 family (Csa-miR156s) and their corresponding target genes in the current study. Bioinformatic approaches were employed to characterize the Csa-miR156s, analyze their expression profiles, and investigate their regulatory relationships with target genes. Additionally, quantitative real-time PCR (qPCR) was used to assess the expression of Csa-miR156s under PEG-simulated drought stress. The results indicated that the miR156 family is divided into four major branches, with the 17 members distributed across them. Fifteen Csa-miR156s were located on five cucumber chromosomes, while the remaining two were repeatedly distributed in chloroplast, mitochondrial, and scaffold sequences. The 17 mature Csa-miR156s were derived from 17 distinct precursor sequences, all of which could form stable stem-loop secondary structures. Analysis of promoter regions revealed that Csa-miR156s contained cis-acting elements associated with responses to light, hormones, or stresses, as well as biosynthesis and metabolism. Expression analyses showed differential expression patterns between Csa-miR156s and their target genes, and qPCR confirmed significant changes in Csa-miR156 expression under drought stress compared with controls. These results suggest that Csa-miR156s are likely involved in the drought stress response in cucumber, thus providing a robust theoretical foundation and offering candidate genes for the future genetic improvement of drought tolerance in cucumber.