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<p>Identification of key genes associated with drought tolerance in <i>chrysanthemum</i> and development of drought-tolerant cultivars via genetic improvement are critical for improving drought resistance and thus promoting yield potential. The TIFY gene family plays an important role in plant hormone signal transduction and stress response. In this study, the differentially expressed gene <i>CmTIFY10A</i>, which was highly expressed in <i>chrysanthemum</i> under drought stress, was identified via transcriptomic analysis. Subcellular localization and yeast transcription activity analysis revealed that CmTIFY10A is a nuclear protein and has no transcriptional self-activation activity in yeast cells. <i>CmTIFY10A-</i>overexpressed and <i>CmTIFY10A-</i>silenced (RNAi) plants were generated via the <i>Agrobacterium</i>-mediated transformation of <i>chrysanthemum</i>. Further analysis revealed that the drought tolerance of <i>chrysanthemum</i> improved after silencing of the <i>CmTIFY10A</i> gene compared with that of the WT plants. Following the measurement and analysis of a series of plant physiological indicators, the contents of osmotic regulators (including proline (Pro) and soluble sugars (Ss)) were significantly increased; the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)) were markedly elevated; Malondialdehyde (MDA) content, relative electrical conductivity (REC), and hydrogen peroxide accumulation were all significantly reduced. Further investigation revealed that under drought stress, <i>CmTIFY10A</i>-silenced transgenic plants exhibited markedly elevated transcription levels of osmotic regulation-related genes (<i>CmP5CS</i>, <i>CmMDH</i>), along with significantly upregulated expression of antioxidant-related genes (<i>CmCu-ZnSOD</i>, <i>CmCAT</i>, <i>CmPOD</i>). These findings indicate that silencing <i>CmTIFY10A</i> exerts a positive regulatory effect on the ROS scavenging system, osmotic adjustment, and membrane protection in chrysanthemums, thereby maintaining cellular homeostasis and playing an important role in the response of chrysanthemum to drought stress.</p>

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The effect of the CmTIFY10A gene on drought tolerance in garden chrysanthemum

  • 期刊论文

  • 发表日期：

    2026年6月22日

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  • Xinyi Zhang,
  • Taotao Hu,
  • Jiaqun Li,
  • Yuting Zhang,
  • Jiajia Bao,
  • Xiaoying Yang,
  • Rui Yan

摘要

Identification of key genes associated with drought tolerance in chrysanthemum and development of drought-tolerant cultivars via genetic improvement are critical for improving drought resistance and thus promoting yield potential. The TIFY gene family plays an important role in plant hormone signal transduction and stress response. In this study, the differentially expressed gene CmTIFY10A, which was highly expressed in chrysanthemum under drought stress, was identified via transcriptomic analysis. Subcellular localization and yeast transcription activity analysis revealed that CmTIFY10A is a nuclear protein and has no transcriptional self-activation activity in yeast cells. CmTIFY10A-overexpressed and CmTIFY10A-silenced (RNAi) plants were generated via the Agrobacterium-mediated transformation of chrysanthemum. Further analysis revealed that the drought tolerance of chrysanthemum improved after silencing of the CmTIFY10A gene compared with that of the WT plants. Following the measurement and analysis of a series of plant physiological indicators, the contents of osmotic regulators (including proline (Pro) and soluble sugars (Ss)) were significantly increased; the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)) were markedly elevated; Malondialdehyde (MDA) content, relative electrical conductivity (REC), and hydrogen peroxide accumulation were all significantly reduced. Further investigation revealed that under drought stress, CmTIFY10A-silenced transgenic plants exhibited markedly elevated transcription levels of osmotic regulation-related genes (CmP5CS, CmMDH), along with significantly upregulated expression of antioxidant-related genes (CmCu-ZnSOD, CmCAT, CmPOD). These findings indicate that silencing CmTIFY10A exerts a positive regulatory effect on the ROS scavenging system, osmotic adjustment, and membrane protection in chrysanthemums, thereby maintaining cellular homeostasis and playing an important role in the response of chrysanthemum to drought stress.