<p>Carbohydrates play a significant role in cut flower quality and in regulating flower opening. This study investigated carbohydrate partitioning between opened flowers and unopened floral buds of cut <i>Oncidesa</i> inflorescences. The inflorescences of <i>Oncidesa</i> Gower Ramsey ‘Honey Angel’ were harvested when about 50% of the flowers were open. On each branch, flowers were separated into lower and upper flowers, and floral buds were numbered from -1 to -5, starting from the base toward the apex. Floral buds were removed from the apex to generate six bud-load treatments, in which all branches within each inflorescence were adjusted to bear 0, 1, 2, 3, 4, or 5 buds per branch. Wilting of initially opened flowers were recorded over a two-week period in a sugar-free vase solution. Carbohydrates in lower flowers, upper flowers, and each bud position were analyzed on Day 0 and Day 14. The wilting percentage of initially opened flowers increased from 18.8% to 70.8% as the number of buds per branch increased from 0 to 5. The fresh weight, dry weight, and total soluble sugars (TSS) of opened flowers decreased after 14 days in the vase, with a greater decline when more buds were retained. The TSS was lower in floral buds than in opened flowers on Day 0, and TSS in floral buds increased upon bud opening. The highest TSS concentration was found in the most recently opened floral bud on Day 14. Moreover, the -1 bud has higher TSS in the inflorescence with only one bud than in those with five buds. This study revealed that the unopened floral buds competed for carbohydrates with the opened flowers, enhancing the wilting of the opened flowers, and the concentration of TSS increased when the buds opened.</p>