Scholarlink

<p>The diverse ploidy level of banana derived from <i>Musa acuminata</i> (AA) and <i>Musa balbisiana</i> (BB) are pivotal for crop improvement. Accurate ploidy determination is essential but hindered by costly, expertise intensive methods like root-tip chromosome counting and flow cytometry. Hence, this study aimed to explore morphological, stomatal and palynological differences between Ney Poovan diploid (NP2) and anther/microspore derived autotetraploid (NP4) for practical ploidy differentiation. Twenty-five morphological traits along with stomatal and palynological attributes were evaluated. Additionally, leaf droopiness traits were compared with flow cytometry. Key findings revealed distinctions in 17 morphological traits using Bonferroni correction, with pronounced leaf droopiness in NP4 as a standout indicator. Stomatal traits corroborated this, with NP4 exhibiting larger stomata and lower density. Furthermore, pollen analysis indicated restored male fertility in NP4 with high pollen production, viability and germination. Flow cytometry validated the reliability of leaf droopiness traits in distinguishing NP4 from NP2. In conclusion, this study offered critical insights for advancing banana breeding programs, with each method contributing to a comprehensive strategy for improving the success of ploidy based breeding efforts.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A Comprehensive study on morphological, stomatal, palynological attributes and flow cytometry for differentiating anther culture derived autotetraploid from ney poovan diploid (AB)

  • 期刊论文

  • 发表日期：

    2026年2月23日

查看全文
  • Ramajayam Devarajan,
  • Sanjay Chetry,
  • Arjun Singh,
  • Sivasankari Ragupathy,
  • Backiyarani Suthanthiram,
  • Mohanasundaram Arumugam,
  • Giribabu Palaniyappan,
  • Dinesh Kumar Agarwal,
  • James Auxcilia,
  • Thirugnanavel Anbazhagan,
  • Kalpana Sathiamoorthy

摘要

The diverse ploidy level of banana derived from Musa acuminata (AA) and Musa balbisiana (BB) are pivotal for crop improvement. Accurate ploidy determination is essential but hindered by costly, expertise intensive methods like root-tip chromosome counting and flow cytometry. Hence, this study aimed to explore morphological, stomatal and palynological differences between Ney Poovan diploid (NP2) and anther/microspore derived autotetraploid (NP4) for practical ploidy differentiation. Twenty-five morphological traits along with stomatal and palynological attributes were evaluated. Additionally, leaf droopiness traits were compared with flow cytometry. Key findings revealed distinctions in 17 morphological traits using Bonferroni correction, with pronounced leaf droopiness in NP4 as a standout indicator. Stomatal traits corroborated this, with NP4 exhibiting larger stomata and lower density. Furthermore, pollen analysis indicated restored male fertility in NP4 with high pollen production, viability and germination. Flow cytometry validated the reliability of leaf droopiness traits in distinguishing NP4 from NP2. In conclusion, this study offered critical insights for advancing banana breeding programs, with each method contributing to a comprehensive strategy for improving the success of ploidy based breeding efforts.