Scholarlink

<p>Persimmon (<i>Diospyros kaki</i>), an economically significant fruit crop, undergoes complex physiological and biochemical changes during fruit development. This study focused on RNA-sequencing analysis across different stages of persimmon fruit development, specifically targeting the expression patterns of an <i>endochitinase</i> gene identified on chromosome 4, which had not been previously annotated in the <i>D. kaki</i> genome. The <i>endochitinase</i> designated as <i>DKAch04aextra</i>, showed a significant increase in expression, accounting for ~ 10% of the raw read counts, coinciding with the onset of fruit ripening in three pollination variant astringency type persimmon cultivars. Interestingly, this pattern of expression was also observed in pollination constant non-astringency type persimmons, indicating a potential universal role in persimmon fruit ripening regardless of astringency characteristics. Comparative analysis with previously assembled persimmon genomes highlighted the need for further annotation within the <i>D. kaki</i> genome to identify unannotated <i>endochitinase</i> potentially relevant to fruit ripening. While the data do not allow definitive conclusions about a regulatory function, the strong expression pattern of <i>DKAch04aextra</i> during ripening stages suggests its putative involvement in physiological changes such as cell wall modification. These findings provide a basis for considering <i>DKAch04aextra</i> as a candidate gene associated with fruit ripening and may inform future studies aimed at improving fruit quality through genetic and postharvest strategies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Dramatic increase of Endochitinase gene expression during persimmon maturation and its potential role in fruit ripening

  • 期刊论文

  • 发表日期：

    2025年10月1日

查看全文
  • Sewon Oh,
  • Seunghyun Ban,
  • Su Hyeon Lee,
  • Ho-Youn Kim,
  • Je Hyeong Jung

摘要

Persimmon (Diospyros kaki), an economically significant fruit crop, undergoes complex physiological and biochemical changes during fruit development. This study focused on RNA-sequencing analysis across different stages of persimmon fruit development, specifically targeting the expression patterns of an endochitinase gene identified on chromosome 4, which had not been previously annotated in the D. kaki genome. The endochitinase designated as DKAch04aextra, showed a significant increase in expression, accounting for ~ 10% of the raw read counts, coinciding with the onset of fruit ripening in three pollination variant astringency type persimmon cultivars. Interestingly, this pattern of expression was also observed in pollination constant non-astringency type persimmons, indicating a potential universal role in persimmon fruit ripening regardless of astringency characteristics. Comparative analysis with previously assembled persimmon genomes highlighted the need for further annotation within the D. kaki genome to identify unannotated endochitinase potentially relevant to fruit ripening. While the data do not allow definitive conclusions about a regulatory function, the strong expression pattern of DKAch04aextra during ripening stages suggests its putative involvement in physiological changes such as cell wall modification. These findings provide a basis for considering DKAch04aextra as a candidate gene associated with fruit ripening and may inform future studies aimed at improving fruit quality through genetic and postharvest strategies.