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<p>Chalcone synthase (VcCHS) in blueberries (<i>Vaccinium Spp</i>) is an important enzyme that regulates anthocyanin synthesis. However, the cis-element structure and regulatory mechanisms regulating the expression of VcCHS remain to be elucidated. In the study, the partial genome sequence of <i>VcCHS</i> was revealed, and its promoter cloned by Tail-PCR. The promoter sequences of 638&#xa0;bp in blueberries’ <i>VcCHS</i> were isolated and identified utilizing Plant CARE, revealing the presence of A-box, ARE, CAAT-box, TATA-box, GT1-motif, and MYB regulatory motifs that offer numerous binding sites for transcription factors. The Y1H technology was applied to analyze whether <i>VcCHS</i> promoter interacted with <i>VcMYB10</i>. The result shows that <i>VcMYB10</i> positively regulates anthocyanin synthesis of blueberries by interacting with the <i>VcCHS</i> promoter. Consequently, the study provides essential information for uncovering novel regulatory mechanisms of anthocyanin synthesis in blueberries.</p>

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Promoter cloning of VcCHS gene from blueberries and selection of its transcription factors

  • 期刊论文

  • 发表日期：

    2025年3月19日

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  • Lei Zhang,
  • Jian-nan Ma

摘要

Chalcone synthase (VcCHS) in blueberries (Vaccinium Spp) is an important enzyme that regulates anthocyanin synthesis. However, the cis-element structure and regulatory mechanisms regulating the expression of VcCHS remain to be elucidated. In the study, the partial genome sequence of VcCHS was revealed, and its promoter cloned by Tail-PCR. The promoter sequences of 638 bp in blueberries’ VcCHS were isolated and identified utilizing Plant CARE, revealing the presence of A-box, ARE, CAAT-box, TATA-box, GT1-motif, and MYB regulatory motifs that offer numerous binding sites for transcription factors. The Y1H technology was applied to analyze whether VcCHS promoter interacted with VcMYB10. The result shows that VcMYB10 positively regulates anthocyanin synthesis of blueberries by interacting with the VcCHS promoter. Consequently, the study provides essential information for uncovering novel regulatory mechanisms of anthocyanin synthesis in blueberries.