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<p>The carotenoid cleavage oxygenases (CCOs), which include 9-<i>cis</i>-epoxycarotenoid dioxygenases (NCEDs) and carotenoid cleavage dioxygenases (CCDs), are essential for the conversion of carotenoids into apocarotenoids. However, a comprehensive genome-wide identification of the CCO gene family in crape myrtle (<i>Lagerstroemia indica</i>) has not yet been conducted. Our investigation identified 15 <i>CCOs</i> in <i>L. indica</i>, which were categorized into 8 <i>LiCCDs</i> and 7 <i>LiNCEDs</i>. Phylogenetic analysis further classified the LiCCOs into 5 distinct groups: CCD1/4/7/8 and NCED. In addition, <i>LiCCOs</i> clustered into the same clades shared similar exon/intron structures and conserved motifs. Syntenic analysis revealed that segmental and tandem duplication events have played a significant role in the expansion of <i>LiCCOs</i>. The regulatory network involving transcription factors (TFs), including bHLH, MYB, and NAC as putative interacting factors, suggested their involvement in the modulation of <i>LiCCO</i> expression. RNA-sequencing analysis revealed that certain <i>LiCCOs</i> exhibit markedly different expression patterns in response to salt stress. Moreover, LiCCOs were involved in the regulation of branching architecture and flower color biosynthesis in <i>L. indica</i>. Gene expression patterns revealed that <i>LiCCOs</i> are significantly affected by mannitol or NaCl stress. The comprehensive genome-wide identification and expression analysis of <i>LiCCOs</i> offer new insights for further studies on the characterization and function.</p>

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Genome-wide identification of CCO gene family in crape myrtle and functional validation of CCO in branching architecture of crape myrtle

  • 期刊论文

  • 发表日期：

    2025年3月12日

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  • Hui Wei,
  • Wenhui Ji,
  • Jinxin Chen,
  • Qianhui Huang,
  • Guoyuan Liu,
  • Bolin Lian,
  • Fei Zhong,
  • Chunmei Yu,
  • Jian Zhang

摘要

The carotenoid cleavage oxygenases (CCOs), which include 9-cis-epoxycarotenoid dioxygenases (NCEDs) and carotenoid cleavage dioxygenases (CCDs), are essential for the conversion of carotenoids into apocarotenoids. However, a comprehensive genome-wide identification of the CCO gene family in crape myrtle (Lagerstroemia indica) has not yet been conducted. Our investigation identified 15 CCOs in L. indica, which were categorized into 8 LiCCDs and 7 LiNCEDs. Phylogenetic analysis further classified the LiCCOs into 5 distinct groups: CCD1/4/7/8 and NCED. In addition, LiCCOs clustered into the same clades shared similar exon/intron structures and conserved motifs. Syntenic analysis revealed that segmental and tandem duplication events have played a significant role in the expansion of LiCCOs. The regulatory network involving transcription factors (TFs), including bHLH, MYB, and NAC as putative interacting factors, suggested their involvement in the modulation of LiCCO expression. RNA-sequencing analysis revealed that certain LiCCOs exhibit markedly different expression patterns in response to salt stress. Moreover, LiCCOs were involved in the regulation of branching architecture and flower color biosynthesis in L. indica. Gene expression patterns revealed that LiCCOs are significantly affected by mannitol or NaCl stress. The comprehensive genome-wide identification and expression analysis of LiCCOs offer new insights for further studies on the characterization and function.