Evaluation of Brassica nigra germplasm for the development of pre-breeding resources to improve Brassicaceae
摘要
The development of resistant cultivars is crucial for managing the significant threat of clubroot disease to Brassica crops worldwide. In the present study, we screened 68 Brassica nigra accessions for resistance test against Plasmodiophora Brassicae strain Yeoncheon. Among these accessions, four accessions (IT-119421, IT-134983, IT-135140, and IT-305086) exhibited higher resistance (HR) with 0% disease severity index (DSI) while two accessions (IT-279240 and IT-279255) showed resistance (R) with 8% DSI based on the disease classification criteria. A similar result was also observed in the case of percent disease incidence. Additionally, total glucosinolate and total phenol content were also evaluated in these accessions. The highest amount of glucosinolate identified in six different accessions which are statistically similar, i.e., IT-119421 (30.67 µmol g− 1), IT-119439 (25.76 µmol g− 1), IT-119414 (25.17 µmol g− 1), IT-119436 (25.02 µmol g− 1), IT-279248 (24.96 µmol g− 1) and IT-119366 (23.62 µmol g− 1). On the other hand, the highest amount of total phenol content was observed in IT-119379, IT-119414, IT-119404, IT-119421, and IT-119436 (1.51, 1.41, 1.31, 1.31, and 1.25 mg GAE g− 1, respectively). However, no significant correlation was found between these compounds and the DSI. The four highly resistant accessions identified demonstrate their potential as genetic resources for breeding clubroot-resistant cultivars.