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<p>Nitrogen is crucial for plant growth and yield. The nitrate transporter gene family plays a key role in N assimilation and utilization in plants. This family is classified into four types: Nitrate Transporter1/Peptide Transporter family, Nitrate Transporter 2 (<i>NRT2</i>), Chloride Channel, and Slow Anion Channel-associated homologues. <i>NRT2</i> is involved in nitrate uptake and transport through the high-affinity transporter system in both animals and plants. In this study, <i>NRT2</i> family members in <i>Brassica rapa</i> and <i>Brassica oleracea</i> were identified and analyzed to determine their potential functions. The analysis of gene structures, protein conserved domains, and evolutionary associations revealed that all members possess at least one major facilitator superfamily motif, and genes clustered together exhibit similar genetic structures. Collinearity analysis demonstrated the presence of <i>NRT2</i> gene associations in <i>B. rapa, Arabidopsis thaliana,</i> and <i>B. oleracea</i>, but not in <i>Oryza sativa</i>. The promoters of <i>BrNRT2</i> and <i>BolNRT2</i> genes contain numerous cis-acting elements associated with hormones and stress, indicating their role in plant development and stress response. Further analysis of <i>BrNRT2</i> family gene expression patterns in various tissues, including flowers, leaves, stamens, pistils, roots, and stems, as well as under abiotic and biotic stress conditions, showed that <i>BrNRT2.7</i> expression is induced by low N, low temperatures, PEG, and soft rot pathogen. Additionally, the overexpressions of <i>BrNRT2.7</i> in yeast resulted in increased survival under drought stress (PEG) but led to increased sensitivity to low temperatures. These results provide a basis for further investigation into the biological functions of the <i>NRT2</i> gene family in <i>B. rapa</i> and <i>B. oleracea</i><Emphasis Type="Underline">.</Emphasis></p>

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Genome-wide identification of nitrate transporter 2 family in brassica rapa and brassica oleracea and exploratory analysis of BrNRT2 in response to stresses

  • 期刊论文

  • 发表日期：

    2025年1月20日

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  • Jiaqi Liu,
  • Chuang Liang,
  • Xueshuai Wang,
  • Ran Gu,
  • Yan Liu,
  • Yaowei Zhang

摘要

Nitrogen is crucial for plant growth and yield. The nitrate transporter gene family plays a key role in N assimilation and utilization in plants. This family is classified into four types: Nitrate Transporter1/Peptide Transporter family, Nitrate Transporter 2 (NRT2), Chloride Channel, and Slow Anion Channel-associated homologues. NRT2 is involved in nitrate uptake and transport through the high-affinity transporter system in both animals and plants. In this study, NRT2 family members in Brassica rapa and Brassica oleracea were identified and analyzed to determine their potential functions. The analysis of gene structures, protein conserved domains, and evolutionary associations revealed that all members possess at least one major facilitator superfamily motif, and genes clustered together exhibit similar genetic structures. Collinearity analysis demonstrated the presence of NRT2 gene associations in B. rapa, Arabidopsis thaliana, and B. oleracea, but not in Oryza sativa. The promoters of BrNRT2 and BolNRT2 genes contain numerous cis-acting elements associated with hormones and stress, indicating their role in plant development and stress response. Further analysis of BrNRT2 family gene expression patterns in various tissues, including flowers, leaves, stamens, pistils, roots, and stems, as well as under abiotic and biotic stress conditions, showed that BrNRT2.7 expression is induced by low N, low temperatures, PEG, and soft rot pathogen. Additionally, the overexpressions of BrNRT2.7 in yeast resulted in increased survival under drought stress (PEG) but led to increased sensitivity to low temperatures. These results provide a basis for further investigation into the biological functions of the NRT2 gene family in B. rapa and B. oleracea.