<p>Durian (<i>Durio zibethinus</i> L.), often referred to as the king of fruits, is widely distributed across southeast Asia. The objective of this study was to assess durian genotypes using plant phenotyping traits and to evaluate their physiological responses to high VPD. Plant morphometric traits were measured using a high-throughput phenotyping platform. Genotypes, such as Chani, Puang Manee, Long Lab Lae, Dang Indo, and Mon Thong, were identified as giant-canopy types (based on plant perimeter, canopy leaf area, and plant volume) when compared with the compact-canopy types (Nok Yib, Black Thorn, and Kradum). Light response curves of net photosynthetic rate in genotypes Long Lab Lae and Puang Manee decreased under 55% RH, while CO₂ response curves of net photosynthetic rate in genotypes Mon Thong and Dang Indo also declined under the same RH condition. Under high VPD, net photosynthetic rate, transpiration rate, stomatal conductance, and intercellular CO₂ concentration were sharply reduced in all genotypes. Based on hierarchical clustering heatmap using Ward’s method, two genotypes (Chani and Black Thorn) were classified as water-deficit tolerant (Cluster I), whereas six genotypes (Puang Manee, Dang Indo, Kradum, Long Lab Lae, Mon Thong, and Nok Yib) were categorized as water-deficit susceptible (Cluster II). Total soluble solid in the pulp fruit of Chani was higher than in cv. Mon Thong in contrast, a smaller amount of pulp weight was demonstrated. The findings could provide useful information to farmers for selecting durian genotypes according to irrigation management and freshwater availability.</p>