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<p>High soil salinity has become a key factor affecting the yield and quality of alfalfa. Calmodulin 1 (<i>CaM1</i>) gene is involved in salinity stress signal transduction, which plays a positive or negative role in regulating plant salinity tolerance. Nevertheless, the specific function of <i>MsCaM1</i> in alfalfa remains unknown. This study showed that the <i>MsCaM1</i> gene contains a complete open reading frame of 450&#xa0;bp, encoding a protein of 149 amino acids. Subcellular localization analysis revealed that the MsCaM1 protein is located to the nucleus. Meanwhile, the expression of <i>MsCaM1</i> gene showed increased trend by salinity treatment in leaves of salinity tolerance variety GN5. The heterologous expression of <i>MsCaM1</i> in <i>Arabidopsis</i> resulted in increased germination energy, germination percentage, plant height, and shoot fresh weight compared with wild-type. Moreover, the shoots of transgenic <i>Arabidopsis</i> plants under NaCl treatment displayed better growth compared to wild-type plants. In transgenic <i>Arabidopsis</i>, the MDA content significantly decreased by NaCl-treated, while the SOD activity remained unchanged, contrasting with the wild-type, where MDA content unchanged and SOD activity decreased. Moreover, the transgenic <i>Arabidopsis</i> plants have lower O<sub>2</sub><sup>−</sup> level under salinity stress compared to wild-type. Furthermore, <i>MsCaM1</i> expression significantly affected the expression of the <i>AtCaM1</i> genes. These results indicate that <i>MsCaM1</i> may act as a positive regulator of growth and salt tolerance in <i>Arabidopsis</i>. These findings could contribute to the understanding of the role of <i>MsCaM1</i> in alfalfa.</p>

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Overexpression of the MsCaM1 gene from alfalfa promoted growth and salinity tolerance in transgenic Arabidopsis

  • 期刊论文

  • 发表日期：

    2026年7月14日

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  • Rugang Yu,
  • Xin Chen,
  • Xinli Pan,
  • Mengru Rong,
  • Lin Yang,
  • Guoliang Wang,
  • Xueling Du

摘要

High soil salinity has become a key factor affecting the yield and quality of alfalfa. Calmodulin 1 (CaM1) gene is involved in salinity stress signal transduction, which plays a positive or negative role in regulating plant salinity tolerance. Nevertheless, the specific function of MsCaM1 in alfalfa remains unknown. This study showed that the MsCaM1 gene contains a complete open reading frame of 450 bp, encoding a protein of 149 amino acids. Subcellular localization analysis revealed that the MsCaM1 protein is located to the nucleus. Meanwhile, the expression of MsCaM1 gene showed increased trend by salinity treatment in leaves of salinity tolerance variety GN5. The heterologous expression of MsCaM1 in Arabidopsis resulted in increased germination energy, germination percentage, plant height, and shoot fresh weight compared with wild-type. Moreover, the shoots of transgenic Arabidopsis plants under NaCl treatment displayed better growth compared to wild-type plants. In transgenic Arabidopsis, the MDA content significantly decreased by NaCl-treated, while the SOD activity remained unchanged, contrasting with the wild-type, where MDA content unchanged and SOD activity decreased. Moreover, the transgenic Arabidopsis plants have lower O2− level under salinity stress compared to wild-type. Furthermore, MsCaM1 expression significantly affected the expression of the AtCaM1 genes. These results indicate that MsCaM1 may act as a positive regulator of growth and salt tolerance in Arabidopsis. These findings could contribute to the understanding of the role of MsCaM1 in alfalfa.