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<p>Seed development in legumes is vital as it determines yield, nutritional quality, and stress resilience, directly impacting global food security and sustainable agriculture. During the seed filling, legumes accumulate proteins like legumins, vicilins collectively known as seed storage proteins. In a previous genome-wide association study, the chickpea gene LOC101504283 (<i>CaSFAM</i> or <i>Cicer arietinum</i> Seed Filling And Maturation), a cytidine de-aminase domain-containing protein, was identified as a potential candidate gene regulating protein storage during seed development. In this study, we have found that <i>CaSFAM</i> shows induction during seed filling and maturation. Moreover, a mutation in MtrunA17Chr8g0390271, the <i>Medicago truncatula</i> orthologue of <i>CaSFAM</i>, impairs seed filling and maturation. Ectopic overexpression of <i>CaSFAM</i> in roots induces the transcription of seed storage protein (SSP) genes, and causes an increase in total protein content in these roots. RNA-sequencing analysis has shown that <i>CaSFAM</i> is an upstream regulator that can control key genes involved in seed development, including the LAFL transcription factor network—comprising <i>LEC1</i>, <i>ABI3</i>, <i>FUS3</i>, and <i>LEC2</i> genes. Apart from seed filling stage, <i>CaSFAM</i> also regulates late maturation hallmarks like <i>LEA</i>, and genes which are known to prevent precocious germination. Overall, we have shown that <i>CaSFAM</i> is involved in seed-specific events of ABA signaling, seed filling, and late maturation in legumes.</p>

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Chickpea ‘Seed Filling and Maturation’ (CaSFAM), a putative cytidine deaminase gene, can regulate key genes for seed development

  • 期刊论文

  • 发表日期：

    2026年7月15日

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  • Prashant Yadav,
  • Asim Kumar Ghosh,
  • Garima Chauhan,
  • Sonali Paul,
  • Swarup K. Parida,
  • Kaustav Bandyopadhyay

摘要

Seed development in legumes is vital as it determines yield, nutritional quality, and stress resilience, directly impacting global food security and sustainable agriculture. During the seed filling, legumes accumulate proteins like legumins, vicilins collectively known as seed storage proteins. In a previous genome-wide association study, the chickpea gene LOC101504283 (CaSFAM or Cicer arietinum Seed Filling And Maturation), a cytidine de-aminase domain-containing protein, was identified as a potential candidate gene regulating protein storage during seed development. In this study, we have found that CaSFAM shows induction during seed filling and maturation. Moreover, a mutation in MtrunA17Chr8g0390271, the Medicago truncatula orthologue of CaSFAM, impairs seed filling and maturation. Ectopic overexpression of CaSFAM in roots induces the transcription of seed storage protein (SSP) genes, and causes an increase in total protein content in these roots. RNA-sequencing analysis has shown that CaSFAM is an upstream regulator that can control key genes involved in seed development, including the LAFL transcription factor network—comprising LEC1, ABI3, FUS3, and LEC2 genes. Apart from seed filling stage, CaSFAM also regulates late maturation hallmarks like LEA, and genes which are known to prevent precocious germination. Overall, we have shown that CaSFAM is involved in seed-specific events of ABA signaling, seed filling, and late maturation in legumes.