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<p>As environmental conditions shift, plants have developed various adaptive mechanisms, including post-translational modifications such as phosphorylation, acetylation, and methylation. Haloacid dehalogenase (HAD) phosphoproteins are phosphatases known to be involved in responses to inorganic phosphate starvation, but their role in dehydration stress remains unexplored. To gain deeper insights into its role in dehydration stress, we conducted a genome-wide analysis to identify, characterize, and study the different isoforms of HAD proteins in <i>Oryza sativa</i>. We identified 13 <i>OsHAD</i> genes and performed in-silico analyses, assessing their physicochemical properties, gene and protein structures, phylogenetic relationships, <i>cis</i>-regulatory elements, and gene ontology. Transcriptome and expression profile analysis were also performed to examine the expression levels of the <i>OsHAD</i> genes. Lastly, we performed subcellular localization of <i>OsHAD-2</i> in <i>Nicotiana tobaccum</i> using confocal microscopy. Our findings provide new insights into the potential functions of HAD proteins and their role in dehydration stress in <i>Oryza sativa</i>.</p>

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Unveiling the role of HAD phosphoproteins in Oryza sativa: genome-wide identification and expression analysis under dehydration stress

  • 期刊论文

  • 发表日期：

    2026年3月16日

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  • Anuradha Pandey,
  • Dipak Gayen

摘要

As environmental conditions shift, plants have developed various adaptive mechanisms, including post-translational modifications such as phosphorylation, acetylation, and methylation. Haloacid dehalogenase (HAD) phosphoproteins are phosphatases known to be involved in responses to inorganic phosphate starvation, but their role in dehydration stress remains unexplored. To gain deeper insights into its role in dehydration stress, we conducted a genome-wide analysis to identify, characterize, and study the different isoforms of HAD proteins in Oryza sativa. We identified 13 OsHAD genes and performed in-silico analyses, assessing their physicochemical properties, gene and protein structures, phylogenetic relationships, cis-regulatory elements, and gene ontology. Transcriptome and expression profile analysis were also performed to examine the expression levels of the OsHAD genes. Lastly, we performed subcellular localization of OsHAD-2 in Nicotiana tobaccum using confocal microscopy. Our findings provide new insights into the potential functions of HAD proteins and their role in dehydration stress in Oryza sativa.