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<p>The development of the CRISPR/Cas technique has revolutionized plant genetics and breeding, enabling precise gene inactivation and modification in model organisms. CRISPR/Cas-based genome editing coupled with neoteric Base and Prime Editing technologies, is transforming plant science. CRISPR/Cas9 has driven significant advances in agronomic trait enhancement, disease resistance, and stress tolerance, becoming a popular and powerful tool in plant research. To address the need for greater precision, Base and Prime Editing techniques have been developed, allowing single nucleotide modifications in genome without causing double-strand breaks. Base Editing enables the exact conversion of single DNA bases, improving accuracy while Prime Editing introduces targeted DNA alterations with greater precision and versatility. Together, these technologies are expanding the possibilities for plant genetic modification, enhancing crop yield, resilience, and sustainability. This review provides an overview of CRISPR/Cas9 application and the recent improvements in precise genome modification, focusing on Base and Prime Editing.</p>

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Genome editing for trait optimization: the role of CRISPR, base editing and prime editing in modern era of plant science

  • 期刊论文

  • 发表日期：

    2025年10月3日

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  • Varadharajan Nareshkumar,
  • S. R. Harish Chandar,
  • Fabio D’Orso,
  • Janarthanan Aparna,
  • Murugavelu Girija Sangari,
  • Ravi Sumega,
  • Chinnusamy Viswanathan,
  • Chinnaswamy Appunu

摘要

The development of the CRISPR/Cas technique has revolutionized plant genetics and breeding, enabling precise gene inactivation and modification in model organisms. CRISPR/Cas-based genome editing coupled with neoteric Base and Prime Editing technologies, is transforming plant science. CRISPR/Cas9 has driven significant advances in agronomic trait enhancement, disease resistance, and stress tolerance, becoming a popular and powerful tool in plant research. To address the need for greater precision, Base and Prime Editing techniques have been developed, allowing single nucleotide modifications in genome without causing double-strand breaks. Base Editing enables the exact conversion of single DNA bases, improving accuracy while Prime Editing introduces targeted DNA alterations with greater precision and versatility. Together, these technologies are expanding the possibilities for plant genetic modification, enhancing crop yield, resilience, and sustainability. This review provides an overview of CRISPR/Cas9 application and the recent improvements in precise genome modification, focusing on Base and Prime Editing.