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<p>The study of pear dormancy mechanism is currently a hot topic in pear production and has significant economic significance for agricultural production. <i>Pyrus pyrifolia. 'Mixue'</i> are known to have high nutritional and commercial value. To better understand the molecular mechanisms of bud dormancy release, we selected the southern sand pear cultivar <i>Pyrus pyrifolia. 'Mixue'</i> as the test material. We explored the respiratory metabolism rate and changes in endogenous hormones of pear flower buds during the dormancy process. Meanwhile, RNA sequencing was used to obtain the gene expression profile of <i>Pyrus pyrifolia. 'Mixue'</i> flower buds, exploring the molecular mechanism of pear flower bud dormancy. The results showed that there were 697 differentially expressed genes (DEGs) in E01vs E02, 665 DEGs in E01 vs E03, 738 DEGs in E01 vs E04, 581 DEGs in E03 vs E02, 664 DEGs in E03 vs E04, 668 genes were differentially expressed in E04 vs E02. KEGG pathway analysis showed that these DEGs were predominantly mapped to Plant-pathogen interaction pathway and Circadian rhythm-plant pathways in the process of dormancy. Furthermore, the genes related to asparagine synthetase (encoding the formation of ADP), glutamate synthetase 1 (encoding NADH, the precursor of starch formation), glutamate dehydrogenase glutamine synthetase leaf isozyme, <i>CCA1</i> gene enriched in the Circadian rhythm-plant pathway and the four genes of the CML (calmodulin like) family (CML38, CML27, CML25, CML23) are significantly different in the process of pear dormancy, which may play an important role in the regulation of pear bud dormancy. In a word, these results will also be of great help in the regulation of endodormancy to cope with climate change and improve fruit production.</p>

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Comparative transcriptomic analysis provides insight into the key regulatory pathways and differentially expressed genes in Pyrus pyrifolia. 'Mixue'

  • 期刊论文

  • 发表日期：

    2025年1月22日

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  • Huiquan Wang,
  • Qinghua Ye,
  • Chunying Liu,
  • Yunyu Shen,
  • Yang Jiang,
  • Xueying Huang,
  • Shaohua Wu

摘要

The study of pear dormancy mechanism is currently a hot topic in pear production and has significant economic significance for agricultural production. Pyrus pyrifolia. 'Mixue' are known to have high nutritional and commercial value. To better understand the molecular mechanisms of bud dormancy release, we selected the southern sand pear cultivar Pyrus pyrifolia. 'Mixue' as the test material. We explored the respiratory metabolism rate and changes in endogenous hormones of pear flower buds during the dormancy process. Meanwhile, RNA sequencing was used to obtain the gene expression profile of Pyrus pyrifolia. 'Mixue' flower buds, exploring the molecular mechanism of pear flower bud dormancy. The results showed that there were 697 differentially expressed genes (DEGs) in E01vs E02, 665 DEGs in E01 vs E03, 738 DEGs in E01 vs E04, 581 DEGs in E03 vs E02, 664 DEGs in E03 vs E04, 668 genes were differentially expressed in E04 vs E02. KEGG pathway analysis showed that these DEGs were predominantly mapped to Plant-pathogen interaction pathway and Circadian rhythm-plant pathways in the process of dormancy. Furthermore, the genes related to asparagine synthetase (encoding the formation of ADP), glutamate synthetase 1 (encoding NADH, the precursor of starch formation), glutamate dehydrogenase glutamine synthetase leaf isozyme, CCA1 gene enriched in the Circadian rhythm-plant pathway and the four genes of the CML (calmodulin like) family (CML38, CML27, CML25, CML23) are significantly different in the process of pear dormancy, which may play an important role in the regulation of pear bud dormancy. In a word, these results will also be of great help in the regulation of endodormancy to cope with climate change and improve fruit production.