Scholarlink

Objective <p>The aim of the current study is assessing the in vitro anti-inflammatory and antioxidant properties of <i>Laurus nobilis</i> methanolic extract (LNME) and to investigate their impacts on experimental oxidative stress in ulcerative colitis caused by acetic acid (AA).</p> Methods <p>The in vitro antioxidant ability of LNME was evaluated using four tests (DPPH, ABTS, FRAP, and CUPRAC). The anti-inflammatory capacity was assessed using the protein denaturation technique, on the basis of total polyphenol measurement. In an in vivo study, 28 rats were equitably divided into four groups: (1) control group, (2) Laurel group: Rats receiving 250&#xa0;mg/kg&#xa0;B.W of LNME, (3) AA group: Rats receiving 2&#xa0;mL/kg&#xa0;B.W of AA (3%) rectally, (4) Laurel + AA: Rats treated by a combination of LNME and AA. Physical, hematological, biochemical, oxidative stress markers, and histological analyses were performed to determine the severity of the colonic inflammation.</p> Results <p>The results showed that the LNME possessed high contents of polyphenols and flavonoids, which exhibited the most excellent antiradical capacity and metal chelating power. The LNME showed a more potent inhibitory impact against the heat-alteration albumin. The intrarectal instillation of AA in rats caused several modifications: disruption of colonic redox status indicated by diminished contents of GPx, SOD, GSH, elevation of MDA, and severe histopathological changes in the colon. Nevertheless, the treatment with LNME during and at post-colitis has notably mitigated some harmful effects of AA, offering protection against colonic oxidative stress.</p> Conclusion <p>The current study suggests that LNME displays anti-inflammatory, and antioxidant potential and a cytoprotective impact supporting its uses to alleviate ulcerative and colonic oxidative stress.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Therapeutic abilities of Laurus nobilis against oxidative stress and ulcerative colitis caused by acetic acid in rats

  • 期刊论文

  • 发表日期：

    2026年5月12日

查看全文
  • Klibet Fahima,
  • Khiari Mohamed,
  • Laouar Amel,
  • Boudersa Nabil,
  • Hamdaoui Nourhene,
  • Meriane Ilhem,
  • Larous Fatima zohra,
  • Ouchenane Sara

摘要

Objective

The aim of the current study is assessing the in vitro anti-inflammatory and antioxidant properties of Laurus nobilis methanolic extract (LNME) and to investigate their impacts on experimental oxidative stress in ulcerative colitis caused by acetic acid (AA).

Methods

The in vitro antioxidant ability of LNME was evaluated using four tests (DPPH, ABTS, FRAP, and CUPRAC). The anti-inflammatory capacity was assessed using the protein denaturation technique, on the basis of total polyphenol measurement. In an in vivo study, 28 rats were equitably divided into four groups: (1) control group, (2) Laurel group: Rats receiving 250 mg/kg B.W of LNME, (3) AA group: Rats receiving 2 mL/kg B.W of AA (3%) rectally, (4) Laurel + AA: Rats treated by a combination of LNME and AA. Physical, hematological, biochemical, oxidative stress markers, and histological analyses were performed to determine the severity of the colonic inflammation.

Results

The results showed that the LNME possessed high contents of polyphenols and flavonoids, which exhibited the most excellent antiradical capacity and metal chelating power. The LNME showed a more potent inhibitory impact against the heat-alteration albumin. The intrarectal instillation of AA in rats caused several modifications: disruption of colonic redox status indicated by diminished contents of GPx, SOD, GSH, elevation of MDA, and severe histopathological changes in the colon. Nevertheless, the treatment with LNME during and at post-colitis has notably mitigated some harmful effects of AA, offering protection against colonic oxidative stress.

Conclusion

The current study suggests that LNME displays anti-inflammatory, and antioxidant potential and a cytoprotective impact supporting its uses to alleviate ulcerative and colonic oxidative stress.