Scholarlink

Object <p>In light of the dimethoate known toxicity, it is imperative to evaluate its impact on lipid profile and its potential genotoxicity of key species like the Mediterranean green crabs.</p> Methods <p>The current study aimed to assess the effect of dimethoate acute toxicity on the fatty acid composition and DNA integrity of <i>C. aestuarii</i>’s hepatopancreas. Alongside a control group, specimens were exposed for 24&#xa0;h to dimethoate sublethal concentrations (50, 100 and 200&#xa0;µg/L).</p> Results <p>An increase in hydrogen peroxide was reported in all DMT-treated groups, resulting in lipid peroxidation exemplified by high levels of lipid hydroperoxides and malondialdehyde relative to the control group. In line with this, a decrease in saturated fatty acids, and an increase in monounsaturated and polyunsaturated fatty acids were observed after dimethoate treatments. In particular, our results showed enhanced levels of omega-3 amounts, especially, eicosapentaenoic (C20:5n3, EPA) and docosahexaenoic (C22:6n3, DHA) acids. Regarding the omega-6 series as well as their precursor, the linoleic acid (C18:2n6, LA), an increase in their levels was recorded specifically under the medium treatment of dimethoate. These alterations of the fatty acid metabolism were supported by a low omega-6/omega-3 ratio. Furthermore, the results also indicate that crabs exposed to dimethoate showed DNA damage, as evidenced by the decrease in DNA concentration, suggesting possible genotoxicity of the product.</p> Conclusion <p>Our study has uncovered an additional mode of action of dimethoate in crustaceans, and highlighted the usefulness of fatty acid analysis as bio-indicators of specific responses to dimethoate toxicity.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Fatty acid content and DNA concentration of the Mediterranean green crab Carcinus aestuarii’s hepatopancreas in relation to dimethoate acute toxicity

  • 期刊论文

  • 发表日期：

    2025年3月6日

查看全文
  • Dalya Belhassen,
  • Safa Bejaoui,
  • Wafa Trabelsi,
  • Boutheina Ben Abdallah,
  • Zeineb Khila,
  • Nejla Soudani

摘要

Object

In light of the dimethoate known toxicity, it is imperative to evaluate its impact on lipid profile and its potential genotoxicity of key species like the Mediterranean green crabs.

Methods

The current study aimed to assess the effect of dimethoate acute toxicity on the fatty acid composition and DNA integrity of C. aestuarii’s hepatopancreas. Alongside a control group, specimens were exposed for 24 h to dimethoate sublethal concentrations (50, 100 and 200 µg/L).

Results

An increase in hydrogen peroxide was reported in all DMT-treated groups, resulting in lipid peroxidation exemplified by high levels of lipid hydroperoxides and malondialdehyde relative to the control group. In line with this, a decrease in saturated fatty acids, and an increase in monounsaturated and polyunsaturated fatty acids were observed after dimethoate treatments. In particular, our results showed enhanced levels of omega-3 amounts, especially, eicosapentaenoic (C20:5n3, EPA) and docosahexaenoic (C22:6n3, DHA) acids. Regarding the omega-6 series as well as their precursor, the linoleic acid (C18:2n6, LA), an increase in their levels was recorded specifically under the medium treatment of dimethoate. These alterations of the fatty acid metabolism were supported by a low omega-6/omega-3 ratio. Furthermore, the results also indicate that crabs exposed to dimethoate showed DNA damage, as evidenced by the decrease in DNA concentration, suggesting possible genotoxicity of the product.

Conclusion

Our study has uncovered an additional mode of action of dimethoate in crustaceans, and highlighted the usefulness of fatty acid analysis as bio-indicators of specific responses to dimethoate toxicity.