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<p>Microalgae are increasingly recognized for their health benefits, with dietary use linked to reduced disease risk. <i>Nannochloropsis oceanica</i>, an oleaginous microalga, is especially valued for its omega-3 fatty acid content, particularly eicosapentaenoic acid (EPA). However, the deoiled biomass left after lipid extraction remains underutilized, despite its nutritional potential. This study investigates the value of deoiled <i>N. oceanica</i> biomass as a sustainable source of essential amino acids. Large-scale cultivation produced biomass with the deoiled fraction containing 14% protein and 39% carbohydrates (dry weight). Protein recovery was optimized using various methods, with ball milling and Ultrasound-Assisted Three-Phase Partitioning (UATPP) proving most effective. Amino acid profiling, conducted after acid and enzymatic hydrolysis, revealed that enzymatic digestion better preserved the full amino acid spectrum, while acid hydrolysis yielded higher concentrations of select amino acids. Both crude protein isolates and their hydrolysates exhibited notable antioxidant activity, underscoring their potential as low-cost bioactive ingredients. These findings support the integration of deoiled microalgal biomass into functional foods and nutraceuticals, advancing sustainable bioprocessing and the value-added utilization of microalgae.</p>

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Valorization of deoiled Nannochloropsis oceanica CASA CC201 biomass: process optimization and biochemical profiling for enhanced bioactive protein recovery

  • 期刊论文

  • 发表日期：

    2026年5月19日

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  • Greeshma Kizhissery,
  • Muthu Arumugam

摘要

Microalgae are increasingly recognized for their health benefits, with dietary use linked to reduced disease risk. Nannochloropsis oceanica, an oleaginous microalga, is especially valued for its omega-3 fatty acid content, particularly eicosapentaenoic acid (EPA). However, the deoiled biomass left after lipid extraction remains underutilized, despite its nutritional potential. This study investigates the value of deoiled N. oceanica biomass as a sustainable source of essential amino acids. Large-scale cultivation produced biomass with the deoiled fraction containing 14% protein and 39% carbohydrates (dry weight). Protein recovery was optimized using various methods, with ball milling and Ultrasound-Assisted Three-Phase Partitioning (UATPP) proving most effective. Amino acid profiling, conducted after acid and enzymatic hydrolysis, revealed that enzymatic digestion better preserved the full amino acid spectrum, while acid hydrolysis yielded higher concentrations of select amino acids. Both crude protein isolates and their hydrolysates exhibited notable antioxidant activity, underscoring their potential as low-cost bioactive ingredients. These findings support the integration of deoiled microalgal biomass into functional foods and nutraceuticals, advancing sustainable bioprocessing and the value-added utilization of microalgae.