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<p>Waste leaves of hull-less pumpkin (<i>Cucurbita pepo</i>) were processed with and without the assistance of enzymes in combined and conventional protocols, respectively, to produce white protein concentrate. Enzymatic treatment of fibrous pulp remained after leaf pressing was applied at conditions determined in optimization experiments. Three commercial mixtures of cell wall degrading enzymes - wide spectrum of carbohydrases, enzyme preparation with dominant β-glucanase activity, and cellulase complex were used. Yield of white protein concentrate produced by all enzyme-assisted protocols was higher in comparison to that from conventional protocol. The most prominent increase of yield, for 340%, was achieved with the assistance of wide spectrum of carbohydrase activities. Cellulase complex enabled production of white protein concentrate with the highest content of protein, 57.20 ± 2.92&#xa0;g/100 g<sub>DM</sub>. Protein concentrate produced with the aid of wide spectrum of carbohydrases had the lowest content of fiber (7.58 ± 0.80&#xa0;g/100 g<sub>DM</sub>) while that from conventional protocol had the lowest ash content, 7.38 ± 0.82&#xa0;g/100 g<sub>DM</sub>. Protein concentrates produced in conventional protocol and with the assistance of cellulase complex exhibited higher <i>in vitro</i> antioxidant activity than those produced with other two enzyme mixtures. FTIR and SDS PAGE revealed differences in the presence and ratios of proteins in white protein concentrates that came as a result of different applied protocols.</p>

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Protein concentrate from waste leaves of hull-less pumpkin (Cucurbita pepo L.): the impact of enzyme-assisted protocol on chemical composition and antioxidant activity

  • 期刊论文

  • 发表日期：

    2026年3月19日

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  • Mirjana Antov,
  • Milica Perović,
  • Nemanja Bojanić,
  • Maja Milošević,
  • Zorica Knežević Jugović

摘要

Waste leaves of hull-less pumpkin (Cucurbita pepo) were processed with and without the assistance of enzymes in combined and conventional protocols, respectively, to produce white protein concentrate. Enzymatic treatment of fibrous pulp remained after leaf pressing was applied at conditions determined in optimization experiments. Three commercial mixtures of cell wall degrading enzymes - wide spectrum of carbohydrases, enzyme preparation with dominant β-glucanase activity, and cellulase complex were used. Yield of white protein concentrate produced by all enzyme-assisted protocols was higher in comparison to that from conventional protocol. The most prominent increase of yield, for 340%, was achieved with the assistance of wide spectrum of carbohydrase activities. Cellulase complex enabled production of white protein concentrate with the highest content of protein, 57.20 ± 2.92 g/100 gDM. Protein concentrate produced with the aid of wide spectrum of carbohydrases had the lowest content of fiber (7.58 ± 0.80 g/100 gDM) while that from conventional protocol had the lowest ash content, 7.38 ± 0.82 g/100 gDM. Protein concentrates produced in conventional protocol and with the assistance of cellulase complex exhibited higher in vitro antioxidant activity than those produced with other two enzyme mixtures. FTIR and SDS PAGE revealed differences in the presence and ratios of proteins in white protein concentrates that came as a result of different applied protocols.