Scholarlink

<p>Bacterial cellulose from SCOBY (BCS) is an extracellular polysaccharide biopolymer produced by microbial fermentation. This study explores BCS production from the by-product of red guava kombucha fermentation. The process yields cellulose membranes that float on the surface, requiring optimization to maximize biosynthesis. Response Surface Methodology (RSM) with Box-Behnken Design (BBD) was applied to enhance production by modifying media composition with key nutrients. The study optimized three parameters: Monosodium glutamate (MSG) (A), citric acid (B), and ethanol (C). The best formulation consisted of 0.100% MSG, 0.150% citric acid, and 0.920% ethanol. The RSM model predicted a BCS yield of 175.40&#xa0;g/L (wet weight), with a thickness of 0.55&#xa0;mm, pH 2.755, dissolved protein 1.343&#xa0;mg/mL, and total reducing sugar of 1516.29&#xa0;mg/L. Experimental results obtained a yield of 164.83&#xa0;g/L, thickness of 0.50&#xa0;mm, pH 2.825, dissolved protein 1.32&#xa0;mg/mL, and total reducing sugar of 1386.67&#xa0;mg/L. This study demonstrates the potential of BCS from red guava kombucha for commercial applications. The RSM approach effectively optimized production, showing strong parameter interactions that enhance cellulose yield, making it a promising raw material source.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Optimizing bacterial cellulose production from red guava kombucha by-products: a box-behnken design approach

  • 期刊论文

  • 发表日期：

    2026年1月23日

查看全文
  • Yati Maryati,
  • Hakiki Melanie,
  • Windri Handayani,
  • Yasman

摘要

Bacterial cellulose from SCOBY (BCS) is an extracellular polysaccharide biopolymer produced by microbial fermentation. This study explores BCS production from the by-product of red guava kombucha fermentation. The process yields cellulose membranes that float on the surface, requiring optimization to maximize biosynthesis. Response Surface Methodology (RSM) with Box-Behnken Design (BBD) was applied to enhance production by modifying media composition with key nutrients. The study optimized three parameters: Monosodium glutamate (MSG) (A), citric acid (B), and ethanol (C). The best formulation consisted of 0.100% MSG, 0.150% citric acid, and 0.920% ethanol. The RSM model predicted a BCS yield of 175.40 g/L (wet weight), with a thickness of 0.55 mm, pH 2.755, dissolved protein 1.343 mg/mL, and total reducing sugar of 1516.29 mg/L. Experimental results obtained a yield of 164.83 g/L, thickness of 0.50 mm, pH 2.825, dissolved protein 1.32 mg/mL, and total reducing sugar of 1386.67 mg/L. This study demonstrates the potential of BCS from red guava kombucha for commercial applications. The RSM approach effectively optimized production, showing strong parameter interactions that enhance cellulose yield, making it a promising raw material source.