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<p>Second-generation bioethanol derived from rice straw faces several challenges, including complex processing stages that involve pretreatment, hydrolysis, and fermentation. Physical, chemical, and physicochemical pretreatments require significant energy, generate chemical waste, and are not environmentally friendly. Current methods for second-generation bioethanol production, such as separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and simultaneous saccharification and co-fermentation (SSCF), remain inefficient due to the separation of enzyme production from hydrolysis and fermentation. This study aims to enhance the efficiency of the production process by integrating fungal pretreatment using <i>Pleurotus ostreatus</i> and consolidated bioprocessing (CBP) using a consortium of <i>Trichoderma reesei</i> and <i>Saccharomyces cerevisiae</i> via a one-pot system. Response surface methodology (RSM) using central composite design (CCD) was employed to optimize bioethanol production. The lignin, cellulose, and hemicellulose contents of rice straw decreased by 31.80%, 6.75%, and 11.65%, respectively, after pretreatment for 21 days. The solids concentration of 34.7% produced the highest bioethanol yield of 27.51&#xa0;mg/g whole pretreated rice straw (WPRS) at 38&#xa0;°C for 192&#xa0;h. Integrating fungal pretreatment and CBP using a microbial consortium resulted in a bioethanol yield of 92.17&#xa0;mg/g rice straw for 29 days. This study introduces a novel method for producing second-generation bioethanol from rice straw that is both more efficient and environmentally friendly. These findings provide a foundation for further research on the industrial-scale development of second-generation bioethanol from rice straw.</p> Graphical abstract <p></p>

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Integrated consolidated bioprocessing with fungal pretreatment for bioethanol production from rice straw using a microbial consortium

  • 期刊论文

  • 发表日期：

    2026年1月8日

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  • Andhika Cahaya Titisan Sukma,
  • Budiyono Budiyono,
  • Ahmad Ni’matullah Al-Baarri

摘要

Second-generation bioethanol derived from rice straw faces several challenges, including complex processing stages that involve pretreatment, hydrolysis, and fermentation. Physical, chemical, and physicochemical pretreatments require significant energy, generate chemical waste, and are not environmentally friendly. Current methods for second-generation bioethanol production, such as separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and simultaneous saccharification and co-fermentation (SSCF), remain inefficient due to the separation of enzyme production from hydrolysis and fermentation. This study aims to enhance the efficiency of the production process by integrating fungal pretreatment using Pleurotus ostreatus and consolidated bioprocessing (CBP) using a consortium of Trichoderma reesei and Saccharomyces cerevisiae via a one-pot system. Response surface methodology (RSM) using central composite design (CCD) was employed to optimize bioethanol production. The lignin, cellulose, and hemicellulose contents of rice straw decreased by 31.80%, 6.75%, and 11.65%, respectively, after pretreatment for 21 days. The solids concentration of 34.7% produced the highest bioethanol yield of 27.51 mg/g whole pretreated rice straw (WPRS) at 38 °C for 192 h. Integrating fungal pretreatment and CBP using a microbial consortium resulted in a bioethanol yield of 92.17 mg/g rice straw for 29 days. This study introduces a novel method for producing second-generation bioethanol from rice straw that is both more efficient and environmentally friendly. These findings provide a foundation for further research on the industrial-scale development of second-generation bioethanol from rice straw.

Graphical abstract