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<p>Biohydrogen production by the dark fermentation (DF) process is an efficient as well as sustainable bioenergy approach. An isolate <i>Escherichia coli</i> SPAUBT [PQ260983] was isolated in this work, and it was used for biohydrogen production using batch fermentation mode. Initially, a 2.5&#xa0;L/L of biohydrogen volume was produced in dark fermentation after single parameter optimization using the isolated bacteria. To enhance hydrogen production, optimization of the fermentation process parameters like substrate concentration, pH, and temperature was carried out using Response Surface Methodology technique (RSM) with the Central Composite Design (CCD) model. Following single- and multi-parameter runs of optimization experiments, the production of hydrogen reached 2.8&#xa0;L/L. Further to inhibit the role of Volatile Fatty Acids (VFAs), biochar was employed. The use of biochar from peanut shells greatly increased hydrogen production up to a maximum production of 3.2&#xa0;L/L. Biochar inhibits the accumulation of VFAs by adsorbing them onto its porous surface, thus maintaining the operational pH. Hence, this research points to the promise of coupling biochar addition with optimized fermentation strategies to drive maximum biohydrogen production.</p>

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Enhanced biohydrogen production from dark fermentation: optimization and Biochar augmentation with the isolated strain

  • 期刊论文

  • 发表日期：

    2026年1月23日

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  • Poulami Sarkar,
  • Soumyajit Chandra,
  • Shah Qamar,
  • Srijoni Banerjee,
  • Soumya Pandit

摘要

Biohydrogen production by the dark fermentation (DF) process is an efficient as well as sustainable bioenergy approach. An isolate Escherichia coli SPAUBT [PQ260983] was isolated in this work, and it was used for biohydrogen production using batch fermentation mode. Initially, a 2.5 L/L of biohydrogen volume was produced in dark fermentation after single parameter optimization using the isolated bacteria. To enhance hydrogen production, optimization of the fermentation process parameters like substrate concentration, pH, and temperature was carried out using Response Surface Methodology technique (RSM) with the Central Composite Design (CCD) model. Following single- and multi-parameter runs of optimization experiments, the production of hydrogen reached 2.8 L/L. Further to inhibit the role of Volatile Fatty Acids (VFAs), biochar was employed. The use of biochar from peanut shells greatly increased hydrogen production up to a maximum production of 3.2 L/L. Biochar inhibits the accumulation of VFAs by adsorbing them onto its porous surface, thus maintaining the operational pH. Hence, this research points to the promise of coupling biochar addition with optimized fermentation strategies to drive maximum biohydrogen production.