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<p>This study explores modifications in sources of organic carbon and strategies for adding them to enhance the metabolite production in <i>Haematococcus pluvialis</i>. Thus, the combined effects of two factors were investigated: the type of single organic carbon source (sodium acetate, AC; molasses, MS; glycerol, GLY) and organic carbon feeding strategy, either single pulse (SPF) or multi-pulse (MPF). This study achieved enhanced biomass yields of <i>H. pluvialis</i> using SPF-GLY and MPF-AC. The incorporation of glycerol and molasses significantly enhanced the biosynthesis of proteins, fatty acids, and astaxanthin. Notably, SPF-GLY achieved the highest astaxanthin content in the biomass (6.89% d.w.). Furthermore, this study reports, for the first time, the activity of the enzyme asparaginase in <i>H. pluvialis</i>. The highest asparaginase activities were observed in SPF-AC and SPF-GLY (245 ± 16&#xa0;IU&#xa0;mg<sup>−1</sup> d.w.). These findings indicate that selecting the appropriate organic carbon source and feeding strategy can optimize metabolite production in <i>H. pluvialis.</i> Moreover, integrating <i>H. pluvialis</i> biomass into biorefinery models could enhance the overall utilisation of the valuable compounds produced.</p> Graphical Abstract <p></p>

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Enhancing high-value metabolite production and asparaginase activity in Haematococcus pluvialis: impact of different organic carbon sources and feeding strategies

  • 期刊论文

  • 发表日期：

    2025年3月25日

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  • Laenne Barbara Silva de Moraes,
  • Géssica Cavalcanti Pereira Mota,
  • Alejandro Molina-Miras,
  • Asterio Sánchez-Mirón,
  • María del Carmen Cerón-García,
  • Alfredo Olivera Gálvez,
  • Ranilson de Souza Bezerra,
  • Francisco García-Camacho

摘要

This study explores modifications in sources of organic carbon and strategies for adding them to enhance the metabolite production in Haematococcus pluvialis. Thus, the combined effects of two factors were investigated: the type of single organic carbon source (sodium acetate, AC; molasses, MS; glycerol, GLY) and organic carbon feeding strategy, either single pulse (SPF) or multi-pulse (MPF). This study achieved enhanced biomass yields of H. pluvialis using SPF-GLY and MPF-AC. The incorporation of glycerol and molasses significantly enhanced the biosynthesis of proteins, fatty acids, and astaxanthin. Notably, SPF-GLY achieved the highest astaxanthin content in the biomass (6.89% d.w.). Furthermore, this study reports, for the first time, the activity of the enzyme asparaginase in H. pluvialis. The highest asparaginase activities were observed in SPF-AC and SPF-GLY (245 ± 16 IU mg−1 d.w.). These findings indicate that selecting the appropriate organic carbon source and feeding strategy can optimize metabolite production in H. pluvialis. Moreover, integrating H. pluvialis biomass into biorefinery models could enhance the overall utilisation of the valuable compounds produced.

Graphical Abstract