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<p>The species of <i>Agave</i> grow on wastelands with minimal water input, and their leaves can be used as feedstock for biofuel production. The study evaluated the potential of leaf biomass of two species and one variety of <i>Agave</i>: <i>A. cantala</i>, <i>A. sisalana</i>, and <i>Agave</i> Bamra Leela as substrates for bioethanol production through compositional analysis, pretreatments, and enzymatic hydrolysis studies. The cellulose content in the leaves of all the species and variety ranged between 27.95 and 31.63%, whereas hemicellulose and lignin contents ranged from 17.25 to 21.66% and 17.25% and 21.66%, respectively. Cellulose solvent and organic solvent lignocellulose fractionation (COSLIF), which uses phosphoric acid, was highly efficient in deconstructing the biomass compared to sodium hydroxide (NaOH), changing different chemical bonds as shown with SEM, XRD, and FTIR studies. The COSLIF pretreated biomass hydrolyzed with 5 filter paper units (FPU) of cellulase along with 10 international units (IU) of <i>β</i>-glucosidase efficiently released 88.13 to 90.23% of glucose, whereas the NaOH pretreated biomass released 59.42 to 64.38% glucose with the use of 60 FPU of cellulase along with 60&#xa0;IU of <i>β</i>-glucosidase after 72&#xa0;h of incubation. Taking the optimum condition standardized for glucose production, the theoretical ethanol yield was calculated through a fermentation reaction using a factor of 1.11 for adding water molecules and 0.5114 regarding the loss of two carbon dioxides during the reaction process. Taking the 88.13% saccharification efficiency in <i>A. sisalana</i>, ethanol yield from 1&#xa0;Mg of raw biomass was calculated to be 224.4 L.</p>

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Efficient release of fermentable glucose from the Agave leaf biomass using phosphoric acid as the pretreating agent for bioethanol production

  • 期刊论文

  • 发表日期：

    2025年3月5日

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  • Debabrata Dash,
  • Nitesh Kumar Mund,
  • Subhransu Nayak,
  • Prasannajit Mishra,
  • Nihar Ranjan Nayak

摘要

The species of Agave grow on wastelands with minimal water input, and their leaves can be used as feedstock for biofuel production. The study evaluated the potential of leaf biomass of two species and one variety of Agave: A. cantala, A. sisalana, and Agave Bamra Leela as substrates for bioethanol production through compositional analysis, pretreatments, and enzymatic hydrolysis studies. The cellulose content in the leaves of all the species and variety ranged between 27.95 and 31.63%, whereas hemicellulose and lignin contents ranged from 17.25 to 21.66% and 17.25% and 21.66%, respectively. Cellulose solvent and organic solvent lignocellulose fractionation (COSLIF), which uses phosphoric acid, was highly efficient in deconstructing the biomass compared to sodium hydroxide (NaOH), changing different chemical bonds as shown with SEM, XRD, and FTIR studies. The COSLIF pretreated biomass hydrolyzed with 5 filter paper units (FPU) of cellulase along with 10 international units (IU) of β-glucosidase efficiently released 88.13 to 90.23% of glucose, whereas the NaOH pretreated biomass released 59.42 to 64.38% glucose with the use of 60 FPU of cellulase along with 60 IU of β-glucosidase after 72 h of incubation. Taking the optimum condition standardized for glucose production, the theoretical ethanol yield was calculated through a fermentation reaction using a factor of 1.11 for adding water molecules and 0.5114 regarding the loss of two carbon dioxides during the reaction process. Taking the 88.13% saccharification efficiency in A. sisalana, ethanol yield from 1 Mg of raw biomass was calculated to be 224.4 L.