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<p>Xylo-oligosaccharides (XOS) are oligosaccharides derived from hemicellulose and classified as non-digestible sugars, which&#xa0;promote beneficial microbiota growth, improve human health, and are utilized widely for their prebiotic properties. This research focused on the optimization conditions for xylanase and XOS production using corncob and wheat bran by <i>Trichoderma harzianum</i> 4FR8 under solid-state fermentation. The highest crude xylanase (173&#xa0;IU/mL) was achieved with a substrate ratio of 8&#xa0;g corncob and 12&#xa0;g wheat bran, pH 6.0, and 70% moisture content over 3&#xa0;days. The highest yield of the&#xa0;crude enzyme was purified using ammonium sulfate precipitation at 40–60% saturation, followed by dialysis and DEAE-cellulose column chromatography. The purified xylanase demonstrated a molecular mass of 18&#xa0;kDa. The <i>K</i><sub><i>m</i></sub> and <i>V</i><sub><i>max</i></sub> values were 6.56&#xa0;mg/mL and 18.29&#xa0;IU/mg, respectively. XOS produced through xylanase hydrolysis of corncob promoted the growth of <i>Lactobacillus lactis</i> TISTR1464 and <i>Bifidobacterium bifidum</i> TISTR2129. Furthermore, XOS from corncob can be utilized for various commercial applications, particularly in the food and nutraceutical industries.</p> Graphic Abstract <p></p>

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Production of xylo-oligosaccharides from corncob using high efficiency xylanase from Trichoderma harzianum 4FR8

  • 期刊论文

  • 发表日期：

    2025年2月11日

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  • Ravinan Chaiyates,
  • Jutaporn Sawaengkaew,
  • Polson Mahakhan

摘要

Xylo-oligosaccharides (XOS) are oligosaccharides derived from hemicellulose and classified as non-digestible sugars, which promote beneficial microbiota growth, improve human health, and are utilized widely for their prebiotic properties. This research focused on the optimization conditions for xylanase and XOS production using corncob and wheat bran by Trichoderma harzianum 4FR8 under solid-state fermentation. The highest crude xylanase (173 IU/mL) was achieved with a substrate ratio of 8 g corncob and 12 g wheat bran, pH 6.0, and 70% moisture content over 3 days. The highest yield of the crude enzyme was purified using ammonium sulfate precipitation at 40–60% saturation, followed by dialysis and DEAE-cellulose column chromatography. The purified xylanase demonstrated a molecular mass of 18 kDa. The Km and Vmax values were 6.56 mg/mL and 18.29 IU/mg, respectively. XOS produced through xylanase hydrolysis of corncob promoted the growth of Lactobacillus lactis TISTR1464 and Bifidobacterium bifidum TISTR2129. Furthermore, XOS from corncob can be utilized for various commercial applications, particularly in the food and nutraceutical industries.

Graphic Abstract