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<p>This study investigates the impact of hydrogen peroxide-assisted ammonia (HAA) pretreatment on reed straw to produce fermentable sugar. Our findings demonstrate that the reaction temperature, quantity of hydrogen peroxide, and ammonia significantly influence the saccharification reaction catalyzed by enzymes. Under these optimal pretreatment conditions (25 wt.% NH<sub>3</sub>·H<sub>2</sub>O, 8 wt.% H<sub>2</sub>O<sub>2</sub>, 170 ℃, 40&#xa0;min), the final sugar yield reaches 59.68% of the theoretical maximum. Furthermore, when treated with a PFI mill at 6000&#xa0;rpm, the total sugar yield from the pretreated straw can reach as high as 81.6%, representing a remarkable increase of 36.70% in sugar yield compared to untreated raw materials under identical conditions. Notably, the dissolved lignin is rich in nitrogen, which can serve as a valuable nutrient for crops. Additionally, it contains highly reactive lignin suitable for high-value applications. Consequently, we assert that HAA pretreatment is an effective method, yielding high quantities of fermentable sugars through enzymatic hydrolysis. It also facilitates the easy utilization of lignin in waste liquid, has a minimal environmental impact, and enables large-scale production feasibility.</p>

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Ammonia combined with hydrogen peroxide pretreatment to improve the production of fermentable sugars in reed

  • 期刊论文

  • 发表日期：

    2025年1月31日

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  • Hui Zhao,
  • Lei Peng,
  • Yehan Tao,
  • Jian Du,
  • Yanna Lv,
  • Jinwen Hu,
  • Jie Lu,
  • Haisong Wang

摘要

This study investigates the impact of hydrogen peroxide-assisted ammonia (HAA) pretreatment on reed straw to produce fermentable sugar. Our findings demonstrate that the reaction temperature, quantity of hydrogen peroxide, and ammonia significantly influence the saccharification reaction catalyzed by enzymes. Under these optimal pretreatment conditions (25 wt.% NH3·H2O, 8 wt.% H2O2, 170 ℃, 40 min), the final sugar yield reaches 59.68% of the theoretical maximum. Furthermore, when treated with a PFI mill at 6000 rpm, the total sugar yield from the pretreated straw can reach as high as 81.6%, representing a remarkable increase of 36.70% in sugar yield compared to untreated raw materials under identical conditions. Notably, the dissolved lignin is rich in nitrogen, which can serve as a valuable nutrient for crops. Additionally, it contains highly reactive lignin suitable for high-value applications. Consequently, we assert that HAA pretreatment is an effective method, yielding high quantities of fermentable sugars through enzymatic hydrolysis. It also facilitates the easy utilization of lignin in waste liquid, has a minimal environmental impact, and enables large-scale production feasibility.