<p>The ongoing development of flexible and wearable devices means that the processes and materials for fabricating stretchable electrodes have been the focus of significant research. We developed a high-performance stretchable conductive film with an elongation of up to 950% by fusing conductive nanofibers and efficient micro-wrinkle-structured films. A large-area micro-wrinkle-structured substrate was fabricated by utilizing the difference in the elastic moduli of Polydimethylsiloxane (PDMS) and Ecoflex. To improve the mechanical performance of the conductive nanofiber film, metal nanofibers were semi-embedded on the surface of the micro-wrinkled structure to strongly bond the film and nanofibers together. The fabrication process of the micro-wrinkle-structured film with the semi-embedded conductive nanofiber network (MWF-SCN) can simply and inexpensively fabricate a large-area micro-wrinkle structure, and the mechanical stability can be significantly improved by semi-embedding highly conductive metal nanofiber networks with an extremely high aspect ratio in the PDMS layer. In addition, the electrical properties were maintained even during repeated bending and stretching. This approach can provide robust conductive networks with improved durability and electrical stability, thus offering promising alternatives for numerous future electronic, optical, display, energy, and sensor devices.</p> Graphical Abstract <p></p>