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<p>The synergistic interplay between biofilm production and carbapenem-resistance-associated (CRA) genes exacerbates the therapeutic challenges of device-related CPE/CPO infections. This research aimed to characterize biofilm-producing CPE/CPO. A total of <i>n</i> = 271 specimens was analyzed by GeneXpert Carba-R Assay and VITEK system for strain characterization, with biofilm production assessed through the Congo Red Agar assay. CPE/CPO strains constituted 12.64% (<i>n</i> = 23) of isolates. The&#xa0;relative abundances were: <i>Klebsiella pneumoniae</i> (38.5%, <i>n</i> = 70), <i>Escherichia coli</i> (30.77%, <i>n</i> = 56), and <i>Pseudomonas aeruginosa</i> (14.8%, <i>n</i> = 27). Least identified were non-CPE/CPO strains <i>Moraxella catarrhalis</i> and <i>Citrobacter werkmanii</i> (0.5%, <i>n</i> = 1 each). <i>Klebsiella pneumoniae</i> was most frequent in sputum and blood, while <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i> predominated in urine and wound swabs, respectively. CRA genes such as OXA-48 (48%), NDM (26%), and a combination of these two genes were predominantly found in the CPE/CPO strains. The highly frequent CRA genes in <i>Klebsiella pneumoniae</i> were OXA-48 (39.13%) and NDM (13.05%). <i>E. coli, Pseudomonas aeruginosa and Acinetobacter baumannii</i> harbor only one of the CRE genes. <i>E. coli</i> harbored the OXA-48 gene; however, <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i> possessed NDM resistance genes. Most (82.6%) of the CPE/CPO strains were moderate to strong biofilm producers. 94.11% of the <i>K</i>. <i>pneumoniae</i> were moderate-strong biofilm producers. OXA-48 and NDM CRA genes in biofilm-producing CPE/CPO necessitate strengthening surveillance and effective infection control policies.</p>

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Molecular Characterization and Relative Abundance of Biofilm-Producing Carbapenemase-Producing Enterobacteriaceae (CPE) or Carbapenemase-Producing Organisms (CPOs) of Clinical Origin in the Jazan, Saudi Arabia

  • 期刊论文

  • 发表日期：

    2025年3月18日

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  • Mohammad Asrar Izhari,
  • Ahmed R. A. Gosady,
  • Ali Alisaac,
  • Abdulmajeed Abdulghani A. Sindi,
  • Mansoor Alsahag,
  • Mansour A. A. Hadadi,
  • Daifallah M. M. Dardari,
  • Talal M. A. Algurashi,
  • Foton E. Alotaibi

摘要

The synergistic interplay between biofilm production and carbapenem-resistance-associated (CRA) genes exacerbates the therapeutic challenges of device-related CPE/CPO infections. This research aimed to characterize biofilm-producing CPE/CPO. A total of n = 271 specimens was analyzed by GeneXpert Carba-R Assay and VITEK system for strain characterization, with biofilm production assessed through the Congo Red Agar assay. CPE/CPO strains constituted 12.64% (n = 23) of isolates. The relative abundances were: Klebsiella pneumoniae (38.5%, n = 70), Escherichia coli (30.77%, n = 56), and Pseudomonas aeruginosa (14.8%, n = 27). Least identified were non-CPE/CPO strains Moraxella catarrhalis and Citrobacter werkmanii (0.5%, n = 1 each). Klebsiella pneumoniae was most frequent in sputum and blood, while Escherichia coli and Pseudomonas aeruginosa predominated in urine and wound swabs, respectively. CRA genes such as OXA-48 (48%), NDM (26%), and a combination of these two genes were predominantly found in the CPE/CPO strains. The highly frequent CRA genes in Klebsiella pneumoniae were OXA-48 (39.13%) and NDM (13.05%). E. coli, Pseudomonas aeruginosa and Acinetobacter baumannii harbor only one of the CRE genes. E. coli harbored the OXA-48 gene; however, Pseudomonas aeruginosa and Acinetobacter baumannii possessed NDM resistance genes. Most (82.6%) of the CPE/CPO strains were moderate to strong biofilm producers. 94.11% of the K. pneumoniae were moderate-strong biofilm producers. OXA-48 and NDM CRA genes in biofilm-producing CPE/CPO necessitate strengthening surveillance and effective infection control policies.