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<p><i>Scepticus tigrinus</i> (Roelofs) (Coleoptera: Curculionidae) and its related species are polyphagous weevil pests that damage various vegetables and crops. Despite their agricultural importance, scalable and straightforward rearing methods using artificial diets have not been established, limiting physiological and developmental studies necessary for effective pest control. The present study evaluated a commercially available artificial diet (F1675) for rearing <i>S. tigrinus</i>. We found that this artificial diet is suitable for the larval growth of <i>S. tigrinus</i>. However, the pupation rate was markedly low. To address this issue, we established a combined-diet-rearing protocol in which larvae are fed with an artificial diet until the final instar and then exposed to raw carrot pieces during the late final instar stage. This method significantly improved pupation success compared to the artificial diet alone (21.68%) and demonstrated higher pupation efficiency relative to labor input than the conventional raw plant-based rearing. Notably, some final instar larvae appeared to have pupated without feeding on the plant material, suggesting that non-nutritive cues—possibly chemical signals from the plants—may trigger pupation. The proposed combined-diet protocol offers a practical approach for mass rearing and facilitates physiologic and genetic studies of <i>S. tigrinus</i> and related weevil pests.</p>

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Laboratory rearing of the broad-nosed weevil Scepticus tigrinus (Coleoptera: Curculionidae) using artificial and plant-based diets

  • 期刊论文

  • 发表日期：

    2025年12月17日

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  • Yoshifumi Araki,
  • Kei Matsubayashi,
  • Toshiya Ando

摘要

Scepticus tigrinus (Roelofs) (Coleoptera: Curculionidae) and its related species are polyphagous weevil pests that damage various vegetables and crops. Despite their agricultural importance, scalable and straightforward rearing methods using artificial diets have not been established, limiting physiological and developmental studies necessary for effective pest control. The present study evaluated a commercially available artificial diet (F1675) for rearing S. tigrinus. We found that this artificial diet is suitable for the larval growth of S. tigrinus. However, the pupation rate was markedly low. To address this issue, we established a combined-diet-rearing protocol in which larvae are fed with an artificial diet until the final instar and then exposed to raw carrot pieces during the late final instar stage. This method significantly improved pupation success compared to the artificial diet alone (21.68%) and demonstrated higher pupation efficiency relative to labor input than the conventional raw plant-based rearing. Notably, some final instar larvae appeared to have pupated without feeding on the plant material, suggesting that non-nutritive cues—possibly chemical signals from the plants—may trigger pupation. The proposed combined-diet protocol offers a practical approach for mass rearing and facilitates physiologic and genetic studies of S. tigrinus and related weevil pests.