<p>The zoophytophagous mirid <i>Nesidiocoris tenuis</i> (Reuter) (Hemiptera: Miridae) is an important biological control agent that primarily acts against small insect pests of tomato (<i>Solanum lycopersicum</i> L., Solanales: Solanaceae). In Japan, biological control programs in vegetable greenhouses are primarily implemented using <i>N. tenuis</i> and cleome (<i>Cleome hassleriana</i> Chod., Brassicales: Cleomaceae), which is a suitable banker plant for maintaining this mirid. However, the preference of <i>N. tenuis</i> for crop or banker plant remains unclear. Here, we assayed the plant preference of <i>N. tenuis</i> between tomato and cleome using three colonies—individuals reproduced on <i>Crassula ovata</i> (Mill.) Druce (Saxifragales: Crassulaceae), tomato, and cleome. Moreover, we evaluated the effect of temporary (24 h) plant experience on the preference of <i>N. tenuis</i>. The plant preference was assayed by a dual-choice test using potted plants. The results showed that individuals reproduced on <i>C. ovata</i> showed equal preference for tomato and cleome, suggesting that <i>N. tenuis</i> has no innate preference for either of these plants. In contrast, individuals reproduced on tomato or cleome had a strong preference for the plant species on which they were grown. Furthermore, individuals who were temporarily exposed to a plant were more likely to prefer that plant. Our findings demonstrate that the preference of <i>N. tenuis</i> between tomato and cleome changes reversibly through learning, indicating that plant preference may be artificially manipulated. This study can help develop effective release strategies for <i>N. tenuis</i>, leading to improved banker plant systems for biological control.</p>